Protective Effects of Passiflora Incarnata on Ovarian Ischemia/reperfusion Damage in Rats with Ovarian Torsion

Overview

Journal Ulus Travma Acil Cerrahi Derg

Specialties Emergency Medicine
General Surgery

Date 2023 Dec 11

PMID 38073455

Authors

Serkan Arslan

Firat Asir

Ebru Gokalp Ozkorkmaz

Mustafa Azizoglu

Erol Basuguy

Mehmet Hanifi Okur

Serap Mutlu Ozcelik Otcu

Musemma Alagoz Karabel

Ibrahim Kaplan

Bahattin Aydogdu

Affiliations

Soon will be listed here.

Abstract

Background: This study aimed to investigate whether Passiflora Incarnata (PI) has a protective effect against ischemia-reperfu-sion (IR)-induced oxidative and inflammatory ovarian damage.

Methods: The effects of PI on ovarian ischemia-reperfusion injury were investigated in female Wistar albino rats. The animals were randomly divided into three groups: Group 1 (sham), Group 2 (IR), and Group 3 (IR+PI).

Results: The mean levels of Malondialdehyde (MDA), Myeloperoxidase (MPO), and Total Oxidant Status (TOS) were higher in the IR group (p=0.025, p<0.001, and p=0.016, respectively). The Total Antioxidant Status (TAS) levels were lower in the IR group (p=0.005). Immunostaining revealed significant differences across the groups for Tumor necrosis factor-alpha (TNF-α): 13.84%, 49.51%, and 22.51% for Groups 1, 2, and 3, respectively (p<0.01). Bax: 10.53%, 46.74%, and 26.46% for Groups 1, 2, and 3, respectively (p<0.01). Annexin V: 12.24%, 44.86%, and 23.28% for Groups 1, 2, and 3, respectively (p<0.01). The mean scores for hemorrhage, inflammation, follicular degeneration, and congestion showed significant variations among the groups, all registering p<0.001.

Conclusion: Passiflora Incarnata exhibited antioxidant, anti-inflammatory, and anti-apoptotic properties, promoting cell survival, histologically protecting ovarian tissue, and ameliorating IR injury by reducing oxidative stress.

References

Kula H, Ilgen O, Kurt S, Yilmaz F . Effects of allium cepa on ovarian torsion-detorsion injury in a rat model. Turk J Obstet Gynecol. 2023; 20(2):137-141. PMC: 10236230. DOI: 10.4274/tjod.galenos.2023.41763. View

Kalyoncu S, Yilmaz B, Demir M, Tuncer M, Bozdag Z, Ince O . Melatonin attenuates ovarian ischemia reperfusion injury in rats by decreasing oxidative stress index and peroxynitrite. Turk J Med Sci. 2020; 50(6):1513-1522. PMC: 7605088. DOI: 10.3906/sag-2004-135. View

Ozkisacik S, Yazici M, Gursoy H, Culhaci N . Does gradual detorsion protect the ovary against ischemia-reperfusion injury in rats?. Pediatr Surg Int. 2014; 30(4):437-40. DOI: 10.1007/s00383-014-3480-3. View

Paglia D, Valentine W . Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1):158-69. View

Crowe A, Yue W . Semi-quantitative Determination of Protein Expression using Immunohistochemistry Staining and Analysis: An Integrated Protocol. Bio Protoc. 2019; 9(24). PMC: 6924920. DOI: 10.21769/BioProtoc.3465. View

Sasaki K, Miller C . Adnexal torsion: review of the literature. J Minim Invasive Gynecol. 2013; 21(2):196-202. DOI: 10.1016/j.jmig.2013.09.010. View

Eser A, Hizli D, Haltas H, Namuslu M, Kosus A, Kosus N . Effects of curcumin on ovarian ischemia-reperfusion injury in a rat model. Biomed Rep. 2015; 3(6):807-813. PMC: 4660605. DOI: 10.3892/br.2015.515. View

Agarwal A, Aponte-Mellado A, Premkumar B, Shaman A, Gupta S . The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol. 2012; 10:49. PMC: 3527168. DOI: 10.1186/1477-7827-10-49. View

Amini F, Amini-Khoei H, Haratizadeh S, Setayesh M, Basiri M, Raeiszadeh M . Hydroalcoholic extract of Passiflora incarnata improves the autistic-like behavior and neuronal damage in a valproic acid-induced rat model of autism. J Tradit Complement Med. 2023; 13(4):315-324. PMC: 10310915. DOI: 10.1016/j.jtcme.2023.02.005. View

10.

Esterbauer H, Cheeseman K . Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990; 186:407-21. DOI: 10.1016/0076-6879(90)86134-h. View

11.

Janda K, Wojtkowska K, Jakubczyk K, Antoniewicz J, Skonieczna-Zydecka K . in Neuropsychiatric Disorders-A Systematic Review. Nutrients. 2020; 12(12). PMC: 7766837. DOI: 10.3390/nu12123894. View

12.

Osmanlioglu S, Arslan M, Dag R, Yigman Z, Ceyhan M, Er F . Artemisinin reduces acute ovarian ischemia-reperfusion injury in rats. Reprod Toxicol. 2023; 119:108417. DOI: 10.1016/j.reprotox.2023.108417. View

13.

Erel O . A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004; 37(2):112-9. DOI: 10.1016/j.clinbiochem.2003.10.014. View

14.

Dasgupta R, Renaud E, Goldin A, Baird R, Cameron D, Arnold M . Ovarian torsion in pediatric and adolescent patients: A systematic review. J Pediatr Surg. 2017; 53(7):1387-1391. DOI: 10.1016/j.jpedsurg.2017.10.053. View

15.

Erel O . A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12):1103-11. DOI: 10.1016/j.clinbiochem.2005.08.008. View

16.

Chinolla-Arellano Z, Banuelos-Rodriguez J, Martinez-Sevilla V, Garcia-Bello J . Complicaciones de la histerectomía total laparoscópica en pacientes de un hospital de alta especialidad. Cir Cir. 2021; 89(3):347-353. DOI: 10.24875/CIRU.20000374. View

17.

Ozgur B, Surer H, Yuceturk C, Karakan T, Ozer E, Ogus E . The protective effect of roflumilast and ibuprofen on testicular ischemia reperfusion injury: An experimental study. Ulus Travma Acil Cerrahi Derg. 2022; 28(6):730-735. PMC: 10443011. DOI: 10.14744/tjtes.2021.01361. View

18.

Uzel K, Lakhno I, Turkler C, Kuzucu M, Yazici G, Mammadov R . Tocilizumab is effective in preventing ovarian injury induced by ischemia- reperfusion in rats. An Acad Bras Cienc. 2023; 95(1):e20220442. DOI: 10.1590/0001-3765202320220442. View

19.

Lagana A, Sofo V, Salmeri F, Palmara V, Triolo O, Terzic M . Oxidative Stress during Ovarian Torsion in Pediatric and Adolescent Patients: Changing The Perspective of The Disease. Int J Fertil Steril. 2016; 9(4):416-23. PMC: 4793162. DOI: 10.22074/ijfs.2015.4598. View

20.

Soumya R, Raj K, Abraham A . Passiflora edulis (var. Flavicarpa) Juice Supplementation Mitigates Isoproterenol-induced Myocardial Infarction in Rats. Plant Foods Hum Nutr. 2021; 76(2):189-195. DOI: 10.1007/s11130-021-00891-x. View