Proton Compared to X-Irradiation Induces Different Protein Profiles in Oral Cancer Cells and Their Derived Extracellular Vesicles

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Dec 9

PMID 38069306

Authors

Inga Solgard Juvkam

Olga Zlygosteva

Mateusz Sitarz

Bernd Thiede

Brita Singers Sorensen

Eirik Malinen

Nina Jeppesen Edin

Tine Merete Soland

Hilde Kanli Galtung

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are membrane-bound particles released from cells, and their cargo can alter the function of recipient cells. EVs from X-irradiated cells have been shown to play a likely role in non-targeted effects. However, EVs derived from proton irradiated cells have not yet been studied. We aimed to investigate the proteome of EVs and their cell of origin after proton or X-irradiation. The EVs were derived from a human oral squamous cell carcinoma (OSCC) cell line exposed to 0, 4, or 8 Gy from either protons or X-rays. The EVs and irradiated OSCC cells underwent liquid chromatography-mass spectrometry for protein identification. Interestingly, we found different protein profiles both in the EVs and in the OSCC cells after proton irradiation compared to X-irradiation. In the EVs, we found that protons cause a downregulation of proteins involved in cell growth and DNA damage response compared to X-rays. In the OSCC cells, proton and X-irradiation induced dissimilar cell death pathways and distinct DNA damage repair systems. These results are of potential importance for understanding how non-targeted effects in normal tissue can be limited and for future implementation of proton therapy in the clinic.

Citing Articles

The Effect of Ionising Radiation on the Properties of Tumour-Derived Exosomes and Their Ability to Modify the Biology of Non-Irradiated Breast Cancer Cells-An In Vitro Study.

Lach M, Wroblewska J, Michalak M, Budny B, Wrotkowska E, Suchorska W Int J Mol Sci. 2025; 26(1.

PMID: 39796230 PMC: 11719956. DOI: 10.3390/ijms26010376.

Proton- compared to X-irradiation leads to more acinar atrophy and greater hyposalivation accompanied by a differential cytokine response.

Juvkam I, Zlygosteva O, Sitarz M, Sorensen B, Aass H, Edin N Sci Rep. 2024; 14(1):22311.

PMID: 39333378 PMC: 11437014. DOI: 10.1038/s41598-024-73110-7.

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