An LcMYB111-LcHY5 Module Differentially Activates an Promoter in Different Litchi Cultivars

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Dec 9

PMID 38069137

Authors

Zhidan Xiao

Jing Wang

Nonghui Jiang

Chao Fan

Xu Xiang

Wei Liu

Affiliations

Soon will be listed here.

Abstract

Flavonol synthase (FLS) is the crucial enzyme of the flavonol biosynthetic pathways, and its expression is tightly regulated in plants. In our previous study, two alleles of and , have been identified in litchi, with extremely early-maturing (EEM) cultivars only harboring , while middle-to-late-maturing (MLM) cultivars only harbor . Here, we overexpressed both alleles in tobacco, and transgenic tobacco produced lighter-pink flowers and showed increased flavonol levels while it decreased anthocyanin levels compared to WT. Two allelic promoters of were identified, with EEM cultivars only harboring proLcFLS-A, while MLM cultivars only harbor proLcFLS-B. One positive and three negative R2R3-MYB transcription regulators of expression were identified, among which only positive regulator showed a consistent expression pattern with , which both have higher expression in EEM than that of MLM cultivars. LcMYB111 were further confirmed to specifically activate proLcFLS-A with MYB-binding element (MBE) while being unable to activate proLcFLS-B with mutated MBE (MBEm). LcHY5 were also identified and can interact with LcMYB111 to promote expression. Our study elucidates the function of and its differential regulation in different litchi cultivars for the first time.

References

Stracke R, Ishihara H, Huep G, Barsch A, Mehrtens F, Niehaus K . Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling. Plant J. 2007; 50(4):660-77. PMC: 1976380. DOI: 10.1111/j.1365-313X.2007.03078.x. View

Falcone Ferreyra M, Rius S, Emiliani J, Pourcel L, Feller A, Morohashi K . Cloning and characterization of a UV-B-inducible maize flavonol synthase. Plant J. 2010; 62(1):77-91. DOI: 10.1111/j.1365-313X.2010.04133.x. View

Canibano E, Bourbousse C, Garcia-Leon M, Garnelo Gomez B, Wolff L, Garcia-Baudino C . DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis. Mol Plant. 2021; 14(6):963-982. DOI: 10.1016/j.molp.2021.03.009. View

Park S, Kim D, Park B, Lee J, Lim S . Molecular and Functional Characterization of Oryza sativa Flavonol Synthase (OsFLS), a Bifunctional Dioxygenase. J Agric Food Chem. 2019; 67(26):7399-7409. DOI: 10.1021/acs.jafc.9b02142. View

Mehrtens F, Kranz H, Bednarek P, Weisshaar B . The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol. 2005; 138(2):1083-96. PMC: 1150422. DOI: 10.1104/pp.104.058032. View

Ramsay N, Glover B . MYB-bHLH-WD40 protein complex and the evolution of cellular diversity. Trends Plant Sci. 2005; 10(2):63-70. DOI: 10.1016/j.tplants.2004.12.011. View

Holton T, Brugliera F, Tanaka Y . Cloning and expression of flavonol synthase from Petunia hybrida. Plant J. 1993; 4(6):1003-10. DOI: 10.1046/j.1365-313x.1993.04061003.x. View

Kim D, Park S, Lee J, Ha S, Lim S . Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter. Int J Mol Sci. 2018; 19(1). PMC: 5796253. DOI: 10.3390/ijms19010309. View

An J, Qu F, Yao J, Wang X, You C, Wang X . The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple. Hortic Res. 2017; 4:17023. PMC: 5461414. DOI: 10.1038/hortres.2017.23. View

10.

Liu C, Chi C, Jin L, Zhu J, Yu J, Zhou Y . The bZip transcription factor HY5 mediates CRY1a-induced anthocyanin biosynthesis in tomato. Plant Cell Environ. 2018; 41(8):1762-1775. DOI: 10.1111/pce.13171. View

11.

Jacobs M, Rubery P . Naturally occurring auxin transport regulators. Science. 1988; 241(4863):346-9. DOI: 10.1126/science.241.4863.346. View

12.

Cavallini E, Matus J, Finezzo L, Zenoni S, Loyola R, Guzzo F . The phenylpropanoid pathway is controlled at different branches by a set of R2R3-MYB C2 repressors in grapevine. Plant Physiol. 2015; 167(4):1448-70. PMC: 4378173. DOI: 10.1104/pp.114.256172. View

13.

Zhang P, Chopra S, Peterson T . A segmental gene duplication generated differentially expressed myb-homologous genes in maize. Plant Cell. 2001; 12(12):2311-2322. PMC: 102220. DOI: 10.1105/tpc.12.12.2311. View

14.

Lin G, Lian Y, Ryu J, Sung M, Park J, Park H . Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli. Protein Expr Purif. 2007; 55(2):287-92. DOI: 10.1016/j.pep.2007.05.013. View

15.

Stracke R, WERBER M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 2001; 4(5):447-56. DOI: 10.1016/s1369-5266(00)00199-0. View

16.

Vu T, Jeong C, Nguyen H, Lee D, Lee S, Kim J . Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L. J Agric Food Chem. 2015; 63(35):7819-29. DOI: 10.1021/acs.jafc.5b02994. View

17.

Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C . Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant Mol Biol. 1994; 24(5):743-55. DOI: 10.1007/BF00029856. View

18.

Xu F, Li L, Zhang W, Cheng H, Sun N, Cheng S . Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba. Mol Biol Rep. 2011; 39(3):2285-96. DOI: 10.1007/s11033-011-0978-9. View

19.

Gangappa S, Botto J . The Multifaceted Roles of HY5 in Plant Growth and Development. Mol Plant. 2016; 9(10):1353-1365. DOI: 10.1016/j.molp.2016.07.002. View

20.

. Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol. 2002; 5(3):218-23. DOI: 10.1016/s1369-5266(02)00256-x. View