Comparative Transcriptome Analysis Reveals Sexually Dimorphic Gene Expression in the Gonads of Li

Overview

Journal Animals (Basel)

Date 2023 Dec 9

PMID 38067041

Authors

Ling Huang

Huan Ye

Huamei Yue

Xiaoqian Leng

Rui Ruan

Hao Du

Chuangju Li

Jinming Wu

Affiliations

Soon will be listed here.

Abstract

Li is an endangered cold-water salmonid fish native to China. This study aimed to identify sex-related genes and biological pathways via gonadal transcriptome sequencing of Li. A total of 167,904 unigenes were identified with an average length of 836 bp and an N50 of 1452 bp, of which 84,977 (50.61%) unigenes were successfully annotated in six major databases. Comparative transcriptome analysis identified 22,864 differentially expressed genes (DEGs), of which 17,231 were up-regulated (male-biased genes, mDEGs) and 5633 were down-regulated (female-biased genes, fDEGs). Several DEGs associated with gonadal development were found through Gene Ontology enrichment analysis, such as , , , , and Signaling pathways related to gonadal development were found to be enriched through analysis using the Kyoto Encyclopedia of Genes and Genomes Pathway database, such as genes involves in base excision repair, the notch signaling pathway, neuroactive ligand-receptor interaction, the VEGF signaling pathway, and the estrogen signaling pathway. In addition, mRNA expression levels of 19 DEGs were determined to validate the reliability of the transcriptomic data by quantitative real-time polymerase chain reaction. These results revealed genes and signaling pathways potentially involved in gonadal development in Li and provided basic molecular data for future research on reproductive regulation and breeding of Li.

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