Comparative Study of Indocyanine Green Fluorescence Imaging in Lung Cancer with Near-Infrared-I/II Windows

Overview

Journal Ann Surg Oncol

Publisher Springer

Specialty Oncology

Date 2023 Dec 8

PMID 38063990

Authors

Jiahui Mi

Changjian Li

Feng Yang

Xiaojing Shi

Zeyu Zhang

Lishuang Guo

Guanchao Jiang

Yun Li

Jun Wang

Fan Yang

Zhenhua Hu

Jian Zhou

Affiliations

Soon will be listed here.

Abstract

Background: We compare the application of intravenous indocyanine green (ICG) fluorescence imaging in lung cancer with near-infrared-I (NIR-I) and near-infrared-II (NIR-II) windows.

Methods: From March to December 2022, we enrolled patients who received an intravenous injection of ICG (5 mg/kg) 1 day before the planned lung cancer surgery. The lung cancer nodules were imaged by NIR-I/II fluorescence imaging systems, and the tumor-to-normal-tissue ratio (TNR) was calculated. In addition, the fluorescence intensity and signal-to-background ratio (SBR) of capillary glass tubes containing ICG covered with different thicknesses of lung tissue were measured by NIR-I/II fluorescence imaging systems.

Results: In this study, 102 patients were enrolled, and the mean age was 59.9 ± 9.2 years. A total of 96 (94.1%) and 98 (96.1%) lung nodules were successfully imaged with NIR-I and NIR-II fluorescence, and the TNR of NIR-II was significantly higher than that of NIR-I (3.9 ± 1.3 versus 2.4 ± 0.6, P < 0.001). In multiple linear regression, solid nodules (P < 0.001) and squamous cell carcinoma (P < 0.001) were independent predictors of a higher TNR of NIR-I/II. When capillary glass tubes were covered with lung tissue whose thickness was more than 2 mm, the fluorescence intensity and the SBR of NIR-II were significantly higher than those of NIR-I.

Conclusions: We verified the feasibility of NIR-II fluorescence imaging in intravenous ICG lung cancer imaging for the first time. NIR-II fluorescence can improve the TNR and penetration depth of lung cancer with promising clinical prospects.

Citing Articles

Recent Advances in Indocyanine Green-Based Probes for Second Near-Infrared Fluorescence Imaging and Therapy.

Hu D, Zha M, Zheng H, Gao D, Sheng Z Research (Wash D C). 2025; 8():0583.

PMID: 39830366 PMC: 11739436. DOI: 10.34133/research.0583.

Progression in Near-Infrared Fluorescence Imaging Technology for Lung Cancer Management.

Chen X, Li Y, Su J, Zhang L, Liu H Biosensors (Basel). 2024; 14(10).

PMID: 39451714 PMC: 11506746. DOI: 10.3390/bios14100501.

NIR-II light in clinical oncology: opportunities and challenges.

Zhang Z, Du Y, Shi X, Wang K, Qu Q, Liang Q Nat Rev Clin Oncol. 2024; 21(6):449-467.

PMID: 38693335 DOI: 10.1038/s41571-024-00892-0.

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