Production and Characterization of Monoclonal Antibodies to Human Tumor Necrosis Factor
Overview
Pathology
Authors
Affiliations
Five stable hybridoma cell lines secreting antibodies to recombinant human tumor necrosis factor (TNF) were established. All monoclonal antibodies belong to the IgG1 subclass. One monoclonal antibody (MAB) (designated as 3B10) neutralizes the L929 cytotoxic activity of natural and recombinant human TNF. Other MABs bind to human TNF but do not neutralize cytotoxic activity. A sandwich enzyme immunoassay specific to human TNF molecule has been developed using 3B10. This assay measures only biologically active human TNF molecules and is as sensitive as a bioassay which measures the cytotoxic activity on L929 cells. These results show that 3B10 is quite useful in studying the biological functions of TNF.
Pharmaceutical aspects of anti-inflammatory TNF-blocking drugs.
Jinesh S Inflammopharmacology. 2015; 23(2-3):71-7.
PMID: 25687751 DOI: 10.1007/s10787-015-0229-0.
Cytokine production by human colonic intraepithelial lymphocytes in controls and ulcerative colitis.
Hoang P, Dalton H, de Silv H, Jewell D Mediators Inflamm. 1994; 3(3):219-22.
PMID: 18472946 PMC: 2367034. DOI: 10.1155/S0962935194000311.
Changes and Relationship of PAF and TNF in Rats with Myocardial Ischaemia and Reperfusion Injury.
Han Y, Li S, Zheng Q, Wang H, Zhang G, Wu Z Mediators Inflamm. 1994; 3(3):205-9.
PMID: 18472943 PMC: 2367036. DOI: 10.1155/S0962935194000281.
Production of polyclonal antibodies to feline tumor necrosis factor.
Otto C, Niagro F, McGraw R, Rawlings C Clin Diagn Lab Immunol. 1997; 4(4):487-90.
PMID: 9220170 PMC: 170556. DOI: 10.1128/cdli.4.4.487-490.1997.
Human tumor necrosis factor increases the resistance against Listeria infection in mice.
Kato K, Nakane A, Minagawa T, Kasai N, Yamamoto K, Sato N Med Microbiol Immunol. 1989; 178(6):337-46.
PMID: 2559307 DOI: 10.1007/BF00197452.