Diversity of Helminths with Zoonotic Potential and Molecular Characterization of Toxocara Canis Infecting Domestic Dogs from Locations of Amazon and Atlantic Forest Brazilian Biomes

Overview

Journal Rev Bras Parasitol Vet

Specialties Parasitology
Veterinary Medicine

Date 2023 Dec 6

PMID 38055439

Authors

Tuan Pedro Dias-Correia

Leandro Batista das Neves

Fernanda Bittencourt-Oliveira

Gabriella Cristina Balzana Giglio

Thiago Cordeiro Pereira

Fernanda Barbosa de Almeida

Rosangela Rodrigues-Silva

Affiliations

Soon will be listed here.

Abstract

The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1.

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