In Vitro and Modelling of Mutant JAK3/STAT5 Signaling in Leukemia

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Dec 6

PMID 38053908

Authors

Sofia A Omari

Hansen J Kosasih

Thomas Chung

Charles E de Bock

Affiliations

Soon will be listed here.

Abstract

Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling. These are subsequently used for the viral transduction of the IL-3 cytokine-dependent Ba/F3 cell line or murine hematopoietic stem and progenitor cells (HSPCs) for and modelling of cytokine-independent growth or leukemia initiation respectively.

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