CpG 684: an Effective Adjuvant for the Inactivated COVID-19 Vaccine in Mice

Overview

Journal Future Virol

Specialty Microbiology

Date 2023 Dec 5

PMID 38051998

Authors

Jiandong Liu

Tianle Cang

Congli Jiang

Kelei Li

Siyuan Liu

Haixin Wang

Meirong Wang

Yan Chen

Yan Shao

Jiankai Liu

Affiliations

Soon will be listed here.

Abstract

Aim: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice.

Materials & Methods: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT.

Results: CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response.

Conclusion: CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine.

References

King K, Larsen B, Gryseels S, Richet C, Kraberger S, Jackson R . Coevolutionary Analysis Implicates Toll-Like Receptor 9 in Papillomavirus Restriction. mBio. 2022; 13(2):e0005422. PMC: 9040848. DOI: 10.1128/mbio.00054-22. View

Sadoff J, Gray G, Vandebosch A, Cardenas V, Shukarev G, Grinsztejn B . Safety and Efficacy of Single-Dose Ad26.COV2.S Vaccine against Covid-19. N Engl J Med. 2021; 384(23):2187-2201. PMC: 8220996. DOI: 10.1056/NEJMoa2101544. View

Yi A, Chang M, Peckham D, Krieg A, Ashman R . CpG oligodeoxyribonucleotides rescue mature spleen B cells from spontaneous apoptosis and promote cell cycle entry. J Immunol. 1998; 160(12):5898-906. View

Romagnani S . Th1/Th2 cells. Inflamm Bowel Dis. 1999; 5(4):285-94. DOI: 10.1097/00054725-199911000-00009. View

Hartmann G, Weiner G, Krieg A . CpG DNA: a potent signal for growth, activation, and maturation of human dendritic cells. Proc Natl Acad Sci U S A. 1999; 96(16):9305-10. PMC: 17777. DOI: 10.1073/pnas.96.16.9305. View

Mistry P, Barmania F, Mellet J, Peta K, Strydom A, Viljoen I . SARS-CoV-2 Variants, Vaccines, and Host Immunity. Front Immunol. 2022; 12:809244. PMC: 8761766. DOI: 10.3389/fimmu.2021.809244. View

Davis H, Weeratna R, Waldschmidt T, Tygrett L, Schorr J, Krieg A . CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. J Immunol. 1998; 160(2):870-6. View

Infante-Duarte C, Kamradt T . Th1/Th2 balance in infection. Springer Semin Immunopathol. 2000; 21(3):317-38. DOI: 10.1007/BF00812260. View

Liu L, Shen L, Liu X, Yu Y, Li Y, Wang L . A safety study of a B-class CpG ODN in Sprague-Dawley rats. J Appl Toxicol. 2011; 32(1):60-71. DOI: 10.1002/jat.1683. View

10.

Azkur A, Akdis M, Azkur D, Sokolowska M, Veen W, Bruggen M . Immune response to SARS-CoV-2 and mechanisms of immunopathological changes in COVID-19. Allergy. 2020; 75(7):1564-1581. PMC: 7272948. DOI: 10.1111/all.14364. View

11.

Pacheco I, Abril N, Morales-Prieto N, Bautista M, Zafra R, Escamilla A . Th1/Th2 balance in the liver and hepatic lymph nodes of vaccinated and unvaccinated sheep during acute stages of infection with Fasciola hepatica. Vet Parasitol. 2017; 238:61-65. DOI: 10.1016/j.vetpar.2017.03.022. View

12.

Polack F, Thomas S, Kitchin N, Absalon J, Gurtman A, Lockhart S . Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine. N Engl J Med. 2020; 383(27):2603-2615. PMC: 7745181. DOI: 10.1056/NEJMoa2034577. View

13.

Liu N, Ohnishi N, Ni L, Akira S, Bacon K . CpG directly induces T-bet expression and inhibits IgG1 and IgE switching in B cells. Nat Immunol. 2003; 4(7):687-93. DOI: 10.1038/ni941. View

14.

. SARS-CoV-2 B.1.1.529 (Omicron) Variant - United States, December 1-8, 2021. MMWR Morb Mortal Wkly Rep. 2021; 70(50):1731-1734. PMC: 8675659. DOI: 10.15585/mmwr.mm7050e1. View

15.

Heger E, Schuetz A, Vasan S . HIV Vaccine Efficacy Trials: RV144 and Beyond. Adv Exp Med Biol. 2018; 1075:3-30. DOI: 10.1007/978-981-13-0484-2_1. View

16.

He B, Qiao X, Cerutti A . CpG DNA induces IgG class switch DNA recombination by activating human B cells through an innate pathway that requires TLR9 and cooperates with IL-10. J Immunol. 2004; 173(7):4479-91. DOI: 10.4049/jimmunol.173.7.4479. View

17.

Yamaguchi K, Hisano M, Isojima S, Irie S, Arata N, Watanabe N . Relationship of Th1/Th2 cell balance with the immune response to influenza vaccine during pregnancy. J Med Virol. 2009; 81(11):1923-8. DOI: 10.1002/jmv.21620. View

18.

Potdar V, Vipat V, Jadhav S, Saha U, Jadhav S, Bhardwaj S . Detection of SARS-CoV-2 variants in India from UK returnees. Infection. 2021; 49(6):1355-1359. PMC: 8220361. DOI: 10.1007/s15010-021-01617-6. View

19.

Tanriover M, Doganay H, Akova M, Guner H, Azap A, Akhan S . Efficacy and safety of an inactivated whole-virion SARS-CoV-2 vaccine (CoronaVac): interim results of a double-blind, randomised, placebo-controlled, phase 3 trial in Turkey. Lancet. 2021; 398(10296):213-222. PMC: 8266301. DOI: 10.1016/S0140-6736(21)01429-X. View

20.

Yang L, Lian Z, Zhang B, Li Z, Zeng L, Li W . Effect of ligustrazine nanoparticles on Th1/Th2 balance by TLR4/MyD88/NF-κB pathway in rats with postoperative peritoneal adhesion. BMC Surg. 2021; 21(1):211. PMC: 8077798. DOI: 10.1186/s12893-021-01201-7. View