The Crystal Structure of the Human Smacovirus 1 Rep Domain

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2023 Dec 5

PMID 38051309

Authors

Lidia K Limon

Ke Shi

Amy Dao

Jacob Rugloski

Kassidy J Tompkins

Hideki Aihara

Wendy R Gordon

Robert L Evans 3rd

Affiliations

Soon will be listed here.

Abstract

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

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