Complicated Target Recognition by Archaeal Box C/D Guide RNAs

Overview

Journal Sci China Life Sci

Specialties Biology
Science

Date 2023 Dec 2

PMID 38041781

Authors

Jiayin Wang

Songlin Wu

Keqiong Ye

Affiliations

Soon will be listed here.

Abstract

Box C/D RNAs guide the site-specific formation of 2'-O-methylated nucleotides (Nm) of RNAs in eukaryotes and archaea. Although C/D RNAs have been profiled in several archaea, their targets have not been experimentally determined. Here, we mapped Nm in rRNAs, tRNAs, and abundant small RNAs (sRNAs) and profiled C/D RNAs in the crenarchaeon Sulfolobus islandicus. The targets of C/D RNAs were assigned by analysis of base-pairing interactions, in vitro modification assays, and gene deletion experiments, revealing a complicated landscape of C/D RNA-target interactions. C/D RNAs widely use dual antisense elements to target adjacent sites in rRNAs, enhancing modification at weakly bound sites. Two consecutive sites can be guided with the same antisense element upstream of box D or D', a phenomenon known as double-specificity that is exclusive to internal box D' in eukaryotic C/D RNAs. Several C/D RNAs guide modification at a single non-canonical site. This study reveals the global landscape of RNA-guided 2'-O-methylation in an archaeon and unexpected targeting rules employed by C/D RNA.

Citing Articles

Landscape of RNA pseudouridylation in archaeon Sulfolobus islandicus.

Li Y, Wu S, Ye K Nucleic Acids Res. 2024; 52(8):4644-4658.

PMID: 38375885 PMC: 11077068. DOI: 10.1093/nar/gkae096.

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