Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition

Overview

Journal Mass Spectrom (Tokyo)

Date 2023 Nov 30

PMID 38034981

Authors

Riko Takata

Yuji Nakabayashi

Kotaro Hashimoto

Akio Miyazato

Issey Osaka

Affiliations

Soon will be listed here.

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist chemical vapor deposition (mist CVD) system was used to produce metal films as ionization assistance materials for SALDI/MS. The system could give Ag film from inexpensive silver trifluoroacetate solution rapidly and simply under atmospheric pressure. Phosphatidylcholines could be detected high sensitively and diacylglycerols (DAGs) could not be detected in MALDI/MS. In the SALDI/MS and the MS imaging with Ag film by mist CVD, both the phosphatidylcholines and the DAGs could be detected and the localized images. In the Ag film-SALDI/MS of lipids, not only Ag-adducted ions but also Na- and K-adducted ions were detected. The Ag film formed by the mist CVD to act as an ionization-assistance material and a cationization agent in SALDI would be useful in MS imaging of biological tissue sections.

Citing Articles

Direct Additive Detection in Polymer Films via Platinum-Assisted SALDI Mass Spectrometry Imaging.

Kawasaki H, Osaka I, Arakawa R Mass Spectrom (Tokyo). 2024; 13(1):A0162.

PMID: 39717495 PMC: 11664312. DOI: 10.5702/massspectrometry.A0162.

Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.

Hashimoto K, Kaneda K, Shimazaki T, Kurihashi C, Yamamoto S, Takata R Mass Spectrom (Tokyo). 2024; 13(1):A0154.

PMID: 39555378 PMC: 11565274. DOI: 10.5702/massspectrometry.A0154.

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