Detection and Characterization of Potential Virulence Determinants in and Strains Isolated from Canine Otitis Externa in Korea

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2023 Nov 30

PMID 38031521

Authors

Gi Yong Lee

Soo In Lee

Ji Heon Park

Sun Do Kim

Geun-Bae Kim

Soo-Jin Yang

Affiliations

Soon will be listed here.

Abstract

Background: A recent increase in the occurrence of canine skin and soft tissue infections, including otitis externa and pyoderma, caused by antimicrobial-resistant and has become a significant public and veterinary health issues.

Objective: We investigated the virulence potentials associated with the occurrence of canine otitis externa in and

Methods: In this study, the prevalence of genes encoding leukocidins, exfoliative toxins, and staphylococcal enterotoxins (SEs) was investigated using previously characterized (n = 26) and (n = 19) isolates derived from canine otitis externa. Susceptibility to cathelicidins (K9CATH and PMAP-36) and hydrogen peroxide (HO) was also examined in both staphylococcal species.

Results: A high prevalence of genes encoding leukocidins (, , and ), exfoliative toxins (, , and ), and SEs was identified in both and isolates. Notably, isolates possessed higher number of SE genes, especially newer SE genes, than isolates harboring clusters. Although no significant differences in susceptibility to K9CATH and HO were observed between the two isolate groups, isolates exhibited enhanced resistance to PMAP-36 compared to isolates.

Conclusions: These findings suggest that high a prevalence of various toxin genes together with enhanced resistance to cathelicidins may contribute to the pathogenicity of and in canine cutaneous infections.

Citing Articles

Prevalence of Bacterial Pathogens Isolated from Canines with Pyoderma and Otitis Externa in Korea: A Systematic Review and Meta-Analysis.

Tanveer M, Ntakiyisumba E, Hirwa F, Yoon H, Oh S, Kim C Vet Sci. 2024; 11(12).

PMID: 39728996 PMC: 11680266. DOI: 10.3390/vetsci11120656.

References

Phumthanakorn N, Fungwithaya P, Chanchaithong P, Prapasarakul N . Enterotoxin gene profile of methicillin-resistant Staphylococcus pseudintermedius isolates from dogs, humans and the environment. J Med Microbiol. 2018; 67(6):866-873. DOI: 10.1099/jmm.0.000748. View

Prevost G, Bouakham T, Piemont Y, Monteil H . Characterisation of a synergohymenotropic toxin produced by Staphylococcus intermedius. FEBS Lett. 1995; 376(3):135-40. DOI: 10.1016/0014-5793(95)01260-9. View

Kang K, Park J, Kim S, Yang S . Potential role of host defense antimicrobial peptide resistance in increased virulence of health care-associated MRSA strains of sequence type (ST) 5 versus livestock-associated and community-associated MRSA strains of ST72. Comp Immunol Microbiol Infect Dis. 2019; 62:13-18. DOI: 10.1016/j.cimid.2018.11.012. View

Abouelkhair M, Bemis D, Giannone R, Frank L, Kania S . Characterization of a leukocidin identified in Staphylococcus pseudintermedius. PLoS One. 2018; 13(9):e0204450. PMC: 6160070. DOI: 10.1371/journal.pone.0204450. View

Cole L . Anatomy and physiology of the canine ear. Vet Dermatol. 2010; 20(5-6):412-21. DOI: 10.1111/j.1365-3164.2009.00849.x. View

Lee S, Kim S, Park J, Yang S . Species Distribution, Antimicrobial Resistance, and Enterotoxigenicity of Non- Staphylococci in Retail Chicken Meat. Antibiotics (Basel). 2020; 9(11). PMC: 7697432. DOI: 10.3390/antibiotics9110809. View

Becker K, Roth R, Peters G . Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J Clin Microbiol. 1998; 36(9):2548-53. PMC: 105160. DOI: 10.1128/JCM.36.9.2548-2553.1998. View

Breathnach R, Quinn P, Baker K, McGeady T, Strobl E, Abbott Y . Association between skin surface pH, temperature and Staphylococcus pseudintermedius in dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis. Vet Dermatol. 2011; 22(4):312-8. DOI: 10.1111/j.1365-3164.2010.00946.x. View

Gomez-Sanz E, Torres C, Lozano C, Saenz Y, Zarazaga M . Detection and characterization of methicillin-resistant Staphylococcus pseudintermedius in healthy dogs in La Rioja, Spain. Comp Immunol Microbiol Infect Dis. 2011; 34(5):447-53. DOI: 10.1016/j.cimid.2011.08.002. View

10.

Futagawa-Saito K, Sugiyama T, Karube S, Sakurai N, Ba-Thein W, Fukuyasu T . Prevalence and characterization of leukotoxin-producing Staphylococcus intermedius in Isolates from dogs and pigeons. J Clin Microbiol. 2004; 42(11):5324-6. PMC: 525193. DOI: 10.1128/JCM.42.11.5324-5326.2004. View

11.

Dicks J, Turnbull J, Russell J, Parkhill J, Alexander S . Genome Sequencing of a Historic Staphylococcus aureus Collection Reveals New Enterotoxin Genes and Sheds Light on the Evolution and Genomic Organization of This Key Virulence Gene Family. J Bacteriol. 2021; 203(10). PMC: 8088605. DOI: 10.1128/JB.00587-20. View

12.

Tanabe T, Toyoguchi M, Hirano F, Chiba M, Onuma K, Sato H . Prevalence of staphylococcal enterotoxins in Staphylococcus pseudintermedius isolates from dogs with pyoderma and healthy dogs. Microbiol Immunol. 2013; 57(9):651-4. DOI: 10.1111/1348-0421.12069. View

13.

Lautz S, Kanbar T, Alber J, Lammler C, Weiss R, Prenger-Berninghoff E . Dissemination of the gene encoding exfoliative toxin of Staphylococcus intermedius among strains isolated from dogs during routine microbiological diagnostics. J Vet Med B Infect Dis Vet Public Health. 2006; 53(9):434-8. DOI: 10.1111/j.1439-0450.2006.00999.x. View

14.

Lin L, Nonejuie P, Munguia J, Hollands A, Olson J, Dam Q . Azithromycin Synergizes with Cationic Antimicrobial Peptides to Exert Bactericidal and Therapeutic Activity Against Highly Multidrug-Resistant Gram-Negative Bacterial Pathogens. EBioMedicine. 2015; 2(7):690-8. PMC: 4534682. DOI: 10.1016/j.ebiom.2015.05.021. View

15.

Naorem R, Goswami G, Gyorgy S, Fekete C . Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions. Sci Rep. 2021; 11(1):18994. PMC: 8460829. DOI: 10.1038/s41598-021-98432-8. View

16.

Yoon J, Lee G, Lee S, Park C, Yoo J, Park H . Prevalence of genes for enterotoxins, toxic shock syndrome toxin 1 and exfoliative toxin among clinical isolates of Staphylococcus pseudintermedius from canine origin. Vet Dermatol. 2010; 21(5):484-9. DOI: 10.1111/j.1365-3164.2009.00874.x. View

17.

Gomez-Sanz E, Torres C, Lozano C, Zarazaga M . High diversity of Staphylococcus aureus and Staphylococcus pseudintermedius lineages and toxigenic traits in healthy pet-owning household members. Underestimating normal household contact?. Comp Immunol Microbiol Infect Dis. 2012; 36(1):83-94. DOI: 10.1016/j.cimid.2012.10.001. View

18.

Otto M . Staphylococcus colonization of the skin and antimicrobial peptides. Expert Rev Dermatol. 2010; 5(2):183-195. PMC: 2867359. DOI: 10.1586/edm.10.6. View

19.

EDWARDS V, Deringer J, Callantine S, Deobald C, Berger P, Kapur V . Characterization of the canine type C enterotoxin produced by Staphylococcus intermedius pyoderma isolates. Infect Immun. 1997; 65(6):2346-52. PMC: 175325. DOI: 10.1128/iai.65.6.2346-2352.1997. View

20.

Santoro D, Bunick D, Graves T, Segre M . Evaluation of canine antimicrobial peptides in infected and noninfected chronic atopic skin. Vet Dermatol. 2013; 24(1):39-47.e10. DOI: 10.1111/j.1365-3164.2012.01091.x. View