Molecular Genotyping of Spp. Isolated from Cheese Samples of Local Stores in Al-Diwaniyah City, Iraq

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2023 Nov 29

PMID 38027405

Authors

Orooba Meteab Faja

Afrah Sabeeh Mhyson

Wisam Reheem Atiyah

Basima Jasim Mohammed

Azal Adnan

Affiliations

Soon will be listed here.

Abstract

Background: Food safety is an important subject that the global cheese industry increases awareness of. This urges these economic sectors to elevate the level of research to minimize cheese contamination with pathogenic bacteria, such as .

Aim: Based on these merits, this study was conducted to genotype spp. isolated from cheese samples of local stores in Al-Diwaniyah City, Iraq.

Methods: The study used 41 samples of local fresh unsalted white cheese in a selective-growth-based isolation of . These isolates were confirmed utilizing a slide-agglutination (SA) test and VITEK 2 system (V2S). Then, the isolates were subjected to conventional PCR and sequencing techniques that both targeted the gene. For subtyping, the isolates were subjected to a random amplified polymorphic DNA (RAPD)-PCR method.

Results: The results of both SA and V2S revealed the presence of 14 (34.2%) isolates of spp. in the cheese samples. The PCR confirmed 6 (42.9%) of these isolates, which further were defined with close nucleotide similarity (98.03%) and (97.88%) to different world isolates, such as subsp. and subsp. , respectively. The RAPD-PCR findings showed different fragments for all the tested isolates.

Conclusion: The present study indicates that the samples of the local fresh unsalted white cheese contain different genotypes, which could be originated from different contamination sources.

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