Targeting B4GALT7 Suppresses the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Through the Cdc2/CyclinB1 and MiR-338-3p/MMP2 Pathway

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2023 Nov 29

PMID 38025683

Authors

Chang Liu

Yuqi Jia

Xinan Zhao

Zifeng Wang

Xiaoxia Zhu

Chan Zhang

Xiaoning Li

Xuhua Zhao

Tao Gong

Hong Zhao

Dong Zhang

Yuhu Niu

Xiushan Dong

Gaopeng Li

Feng Li

Hongwei Zhang

Li Zhang

Jun Xu

Baofeng Yu

Affiliations

Soon will be listed here.

Abstract

Background: As a three-dimensional network involving glycosaminoglycans (GAGs), proteoglycans (PGs) and other glycoproteins, the role of extracellular matrix (ECM) in tumorigenesis is well revealed. Abnormal glycosylation in liver cancer is correlated with tumorigenesis and chemoresistance. However, the role of galactosyltransferase in HCC (hepatocellular carcinoma) is largely unknown.

Methods: Here, the oncogenic functions of B4GALT7 (beta-1,4-galactosyltransferase 7) were identified in HCC by a panel of experiments, including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation, transwell and flow cytometry assay. The expression of B4GALT7 in HCC cell lines and tissues were examined by qPCR (real-time quantitative polymerase chain reaction) and western blot assay. The binding between B4GALT7 and miR-338-3p was examined by dual-luciferase reporter assay.

Results: B4GALT7 encodes galactosyltransferase I and it is highly expressed in HCC cells and human HCC tissues compared with para-tumor specimens. MiR-338-3p was identified to bind the 3' UTR (untranslated region) of B4GALT7. Highly expressed miR-338-3p suppressed HCC cell invasive abilities and rescued the tumor-promoting effect of B4GALT7 in HCC. ShRNA (short hairpin RNA) mediated B4GALT7 suppression reduced HCC cell invasive abilities, and inhibited the expression of MMP-2 and Erk signaling.

Conclusion: These findings identified B4GALT7 as a potential prognostic biomarker and therapeutic target for HCC.

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