Hsa_circ_0001278 Facilitates Colorectal Cancer Progression Sponging MiR-338-5p and Regulating AMOTL1 Expression

Overview

Journal Comb Chem High Throughput Screen

Publisher Bentham Science Publishers

Specialty Chemistry

Date 2023 Nov 29

PMID 38018210

Authors

Hua Ge

Yan Yan

Haomin Wang

Jun Bian

Zhilong Deng

Xian Su

Zaicai Yang

Jiacheng Song

Affiliations

Soon will be listed here.

Abstract

Background: Colorectal cancer (CRC) ranks as the third most common cancer and is second in terms of mortality worldwide. Circular RNAs are involved in the occurrence and development of malignant tumors by functioning either as oncogenes or tumor suppressors.

Method: This study investigated the functions of hsa_circ_0001278 in CRC. We analyzed the expression of hsa_circ_0001278 in CRC tissues and adjacent normal tissues. In order to understand the roles of hsa_circ_0001278 in CRC in terms of cellular biological behavior, in vitro experiments were conducted. A mechanistic study was designed to investigate the regulatory effect of hsa_circ_0001278 on CRC.

Results: Hsa_circ_0001278 was found to be significantly upregulated in CRC specimens. The functional analysis indicated that hsa_circ_0001278 promotes aggressive phenotypes of CRC cells. Further mechanistic studies revealed that hsa_circ_0001278 sponges miR-338-5p to regulate angiomotin-like 1 (AMOTL1), thereby facilitating CRC progression.

Conclusion: Our results demonstrate that hsa_circ_0001278 promotes malignant behaviors in CRC cells by sponging miR-338-5p to regulate AMOTL1 expression. This suggests that hsa_circ_0001278 may serve as a novel target for CRC treatment.

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References

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

Dienstmann R, Vermeulen L, Guinney J, Kopetz S, Tejpar S, Tabernero J . Consensus molecular subtypes and the evolution of precision medicine in colorectal cancer. Nat Rev Cancer. 2017; 17(4):268. DOI: 10.1038/nrc.2017.24. View

Luo Z, Chen X, Zhang Y, Huang Z, Zhao H, Zhao J . Development of a Metastasis-Related Immune Prognostic Model of Metastatic Colorectal Cancer and Its Usefulness to Immunotherapy. Front Cell Dev Biol. 2021; 8:577125. PMC: 7876250. DOI: 10.3389/fcell.2020.577125. View

Agostini M, Mancini M, Candi E . Long non-coding RNAs affecting cell metabolism in cancer. Biol Direct. 2022; 17(1):26. PMC: 9526990. DOI: 10.1186/s13062-022-00341-x. View

Adams B, Anastasiadou E, Esteller M, He L, Slack F . The Inescapable Influence of Noncoding RNAs in Cancer. Cancer Res. 2015; 75(24):5206-10. PMC: 4823153. DOI: 10.1158/0008-5472.CAN-15-1989. View

Wilusz J, Sharp P . Molecular biology. A circuitous route to noncoding RNA. Science. 2013; 340(6131):440-1. PMC: 4063205. DOI: 10.1126/science.1238522. View

Jeck W, Sorrentino J, Wang K, Slevin M, Burd C, Liu J . Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 2012; 19(2):141-57. PMC: 3543092. DOI: 10.1261/rna.035667.112. View

Salzman J, Chen R, Olsen M, Wang P, Brown P . Cell-type specific features of circular RNA expression. PLoS Genet. 2013; 9(9):e1003777. PMC: 3764148. DOI: 10.1371/journal.pgen.1003777. View

Cortes-Lopez M, Miura P . Emerging Functions of Circular RNAs. Yale J Biol Med. 2016; 89(4):527-537. PMC: 5168830. View

10.

Hansen T, Jensen T, Clausen B, Bramsen J, Finsen B, Damgaard C . Natural RNA circles function as efficient microRNA sponges. Nature. 2013; 495(7441):384-8. DOI: 10.1038/nature11993. View

11.

Ruan H, Wang P, Han L . Characterization of circular RNAs with advanced sequencing technologies in human complex diseases. Wiley Interdiscip Rev RNA. 2022; 14(1):e1759. DOI: 10.1002/wrna.1759. View

12.

Sawicki T, Ruszkowska M, Danielewicz A, Niedzwiedzka E, Arlukowicz T, Przybylowicz K . A Review of Colorectal Cancer in Terms of Epidemiology, Risk Factors, Development, Symptoms and Diagnosis. Cancers (Basel). 2021; 13(9). PMC: 8122718. DOI: 10.3390/cancers13092025. View

13.

Zhang Y, Tan X, Lu Y . Exosomal transfer of circ_0006174 contributes to the chemoresistance of doxorubicin in colorectal cancer by depending on the miR-1205/CCND2 axis. J Physiol Biochem. 2021; 78(1):39-50. DOI: 10.1007/s13105-021-00831-y. View

14.

Gu H, Xu Z, Zhang J, Wei Y, Cheng L, Wang J . circ_0038718 promotes colon cancer cell malignant progression via the miR-195-5p/Axin2 signaling axis and also effect Wnt/β-catenin signal pathway. BMC Genomics. 2021; 22(1):768. PMC: 8555003. DOI: 10.1186/s12864-021-07880-z. View

15.

Wu L, Zhang M, Qi L, Zu X, Li Y, Liu L . ERα-mediated alterations in circ_0023642 and miR-490-5p signaling suppress bladder cancer invasion. Cell Death Dis. 2019; 10(9):635. PMC: 6712013. DOI: 10.1038/s41419-019-1827-3. View

16.

Wang Y, Liu J, Ma J, Sun T, Zhou Q, Wang W . Exosomal circRNAs: biogenesis, effect and application in human diseases. Mol Cancer. 2019; 18(1):116. PMC: 6610963. DOI: 10.1186/s12943-019-1041-z. View

17.

Xing Z, Wang R, Wang X, Liu J, Zhang M, Feng K . CircRNA circ-PDCD11 promotes triple-negative breast cancer progression via enhancing aerobic glycolysis. Cell Death Discov. 2021; 7(1):218. PMC: 8380247. DOI: 10.1038/s41420-021-00604-y. View

18.

Yang F, Xuan G, Chen Y, Cao L, Zhao M, Wang C . MicroRNAs Are Key Molecules Involved in the Gene Regulation Network of Colorectal Cancer. Front Cell Dev Biol. 2022; 10:828128. PMC: 9023807. DOI: 10.3389/fcell.2022.828128. View

19.

Wei X, Zhu J, Zhang Y, Zhao Q, Wang H, Gu K . miR-338-5p-ZEB2 axis in Diagnostic, Therapeutic Predictive and Prognostic Value of Gastric Cancer. J Cancer. 2021; 12(22):6756-6772. PMC: 8518007. DOI: 10.7150/jca.58249. View

20.

Han L, Cui D, Li B, Xu W, Lam A, Chan K . MicroRNA-338-5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id-1. Cancer Sci. 2019; 110(12):3677-3688. PMC: 6890449. DOI: 10.1111/cas.14220. View