Harnessing Multiplex CrRNA Enables an Amplification-free/CRISPR-Cas12a-based Diagnostic Methodology for

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2023 Nov 28

PMID 38014967

Authors

Huarui Zhang

Huijuan Zhao

Lu Cao

Bin Yu

Junhong Wei

Guoqing Pan

Jialing Bao

Zeyang Zhou

Affiliations

Soon will be listed here.

Abstract

The multiplex-crRNA CRISPR/Cas12a detection method saves hands-on time, reduces the risk of aerosol pollution, and can be directly applied to detecting silkworms infected with . This study provides a new approach for the inspection and quarantine of silkworm pébrine disease in sericulture and provides a new method for the detection of other pathogens.

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