Generation of Mutants from the 57B Region of

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Nov 25

PMID 38002990

Authors

Eva Louise Steinmetz

Sandra Noh

Christine Kloppel

Martin F Fuhr

Nicole Bach

Mona Evelyn Raffael

Kirsten Hildebrandt

Fabienne Wittling

Doris Jann

Uwe Walldorf

Affiliations

Soon will be listed here.

Abstract

The 57B region of includes a cluster of the three homeobox genes (), (), and (). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for , , and . With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of , which could now be used to further explore the functions of the corresponding genes in the future.

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