Biogenic Selenium Nanoparticles Synthesized with Alginate Oligosaccharides Alleviate Heat Stress-Induced Oxidative Damage to Organs in Broilers Through Activating Nrf2-Mediated Anti-Oxidation and Anti-Ferroptosis Pathways

Overview

Journal Antioxidants (Basel)

Date 2023 Nov 25

PMID 38001826

Authors

Xue-Qing Ye

Yan-Ru Zhu

Yu-Ying Yang

Sheng-Jian Qiu

Wen-Chao Liu

Affiliations

Soon will be listed here.

Abstract

Selenium (Se) is an essential trace element for maintaining health due to its ideal antioxidant properties. We previously prepared a new type of biogenic selenium nanoparticles based on alginate oligosaccharides (SeNPs-AOS), and this study aimed to investigate the protective effects of SeNPs-AOS (Se particle size = 80 nm, Se content = 8%) on organ health in broilers challenged with HS. A total of 192 21-day-old Arbor Acres broilers were randomly divided into four groups according to a 2 × 2 experimental design, including a thermoneutral zone group (TN, raised under 23 ± 1.5 °C); TN + SeNPs-AOS group (TN group supplemented 5 mg/kg SeNPS-AOS); HS group (HS, raised under 33 ± 2 °C for 10 h/day); and HS + SeNPs-AOS group (HS group supplemented 5 mg/kg SeNPS-AOS). There were six replicates in each group (eight broilers per replicate). The results showed that SeNPs-AOS improved the splenic histomorphology, enhanced the activity of catalase (CAT) and glutathione peroxidase (GSH-Px) of the spleen, as well as upregulating the splenic mRNA expression of antioxidant-related genes in broilers under HS. In addition, SeNPs-AOS reversed the pathological changes in bursa caused by HS increased the activity of GST, GSH-Px, and CAT and upregulated the mRNA expression of and antioxidant-related genes in the bursa of heat-stressed broilers. In addition, dietary SeNPs-AOS improved the hepatic damage, increased the activity of GSH-Px in the liver, and upregulated the mRNA expression of antioxidant-related genes while downregulating the gene expression of the liver in broilers during HS. Moreover, dietary SeNPs-AOS upregulated the anti-ferroptosis-related genes expression of liver in broilers under HS. In conclusion, dietary SeNPs-AOS could relieve HS-induced oxidative damage to the spleen, bursa of Fabricius and liver in broilers by upregulating the Nrf2-mediated antioxidant gene expression and SeNPs-AOS could also upregulate the expression of hepatic Nrf2-related anti-ferroptosis genes in heat-stressed broilers. These findings are beneficial for the development of new nano-antioxidants in broilers.

Citing Articles

Phlorotannin Alleviates Liver Injury by Regulating Redox Balance, Apoptosis, and Ferroptosis of Broilers under Heat Stress.

Zhao Z, Yuan Y, Zhao Z, Yao Q, Ye X, Wang Y Antioxidants (Basel). 2024; 13(9).

PMID: 39334707 PMC: 11428727. DOI: 10.3390/antiox13091048.

Nanomedicines Targeting Ferroptosis to Treat Stress-Related Diseases.

Kang H, Meng F, Liu F, Xie M, Lai H, Li P Int J Nanomedicine. 2024; 19:8189-8210.

PMID: 39157732 PMC: 11328858. DOI: 10.2147/IJN.S476948.

Protective effects of sodium humate and its zinc and selenium chelate on the oxidative stress, inflammatory, and intestinal barrier damage of Salmonella Typhimurium-challenged broiler chickens.

Fan Y, Zhou W, Li G, Liu X, Zhong P, Liu K Poult Sci. 2024; 103(5):103541.

PMID: 38471228 PMC: 11067757. DOI: 10.1016/j.psj.2024.103541.

References

Miao Q, Si X, Xie Y, Chen L, Liu Z, Liu L . Effects of acute heat stress at different ambient temperature on hepatic redox status in broilers. Poult Sci. 2020; 99(9):4113-4122. PMC: 7598013. DOI: 10.1016/j.psj.2020.05.019. View

Chen G, Song C, Pantopoulos K, Wei X, Zheng H, Luo Z . Mitochondrial oxidative stress mediated Fe-induced ferroptosis via the NRF2-ARE pathway. Free Radic Biol Med. 2022; 180:95-107. DOI: 10.1016/j.freeradbiomed.2022.01.012. View

Dalto D, Lapointe J, Matte J . Assessment of antioxidative and selenium status by seleno-dependent glutathione peroxidase activity in different blood fractions using a pig model: issues for clinical nutrition and research. J Anim Physiol Anim Nutr (Berl). 2017; 102(1):184-193. DOI: 10.1111/jpn.12677. View

Lee Y, Cho I, Kim J, Lee M, Ku S, Choi J . Hepatoprotective effects of blue honeysuckle on CCl-induced acute liver damaged mice. Food Sci Nutr. 2019; 7(1):322-338. PMC: 6341158. DOI: 10.1002/fsn3.893. View

Chauhan S, Rashamol V, Bagath M, Sejian V, Dunshea F . Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration. Int J Biometeorol. 2021; 65(7):1231-1244. DOI: 10.1007/s00484-021-02083-3. View

Cao X, Guo L, Zhou C, Huang C, Li G, Zhuang Y . Effects of N-acetyl-l-cysteine on chronic heat stress-induced oxidative stress and inflammation in the ovaries of growing pullets. Poult Sci. 2022; 102(1):102274. PMC: 9673114. DOI: 10.1016/j.psj.2022.102274. View

Liu Y, Yin S, Tang J, Liu Y, Jia G, Liu G . Hydroxy Selenomethionine Improves Meat Quality through Optimal Skeletal Metabolism and Functions of Selenoproteins of Pigs under Chronic Heat Stress. Antioxidants (Basel). 2021; 10(10). PMC: 8533020. DOI: 10.3390/antiox10101558. View

Turanov A, Xu X, Carlson B, Yoo M, Gladyshev V, Hatfield D . Biosynthesis of selenocysteine, the 21st amino acid in the genetic code, and a novel pathway for cysteine biosynthesis. Adv Nutr. 2012; 2(2):122-8. PMC: 3065758. DOI: 10.3945/an.110.000265. View

Alian H, Samy H, Ibrahim M, Mahmoud M . Nanoselenium effect on growth performance, carcass traits, antioxidant activity, and immune status of broilers. Environ Sci Pollut Res Int. 2020; 27(31):38607-38616. DOI: 10.1007/s11356-020-09952-1. View

10.

Jung Y, Choi H, Oh E . Selenium mitigates ferroptosis-mediated dopaminergic cell death by regulating the Nrf2/GPX4 pathway. Neurosci Lett. 2023; 810:137314. DOI: 10.1016/j.neulet.2023.137314. View

11.

Ingold I, Berndt C, Schmitt S, Doll S, Poschmann G, Buday K . Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis. Cell. 2018; 172(3):409-422.e21. DOI: 10.1016/j.cell.2017.11.048. View

12.

Lu S, Na K, Wei J, Zhang L, Guo X . Alginate oligosaccharides: The structure-function relationships and the directional preparation for application. Carbohydr Polym. 2022; 284:119225. DOI: 10.1016/j.carbpol.2022.119225. View

13.

Dalgaard T, Briens M, Engberg R, Lauridsen C . The influence of selenium and selenoproteins on immune responses of poultry and pigs. Anim Feed Sci Technol. 2020; 238:73-83. PMC: 7173062. DOI: 10.1016/j.anifeedsci.2018.01.020. View

14.

Liu W, Ou B, Liang Z, Zhang R, Zhao Z . Algae-derived polysaccharides supplementation ameliorates heat stress-induced impairment of bursa of Fabricius via modulating NF-κB signaling pathway in broilers. Poult Sci. 2021; 100(8):101139. PMC: 8264154. DOI: 10.1016/j.psj.2021.101139. View

15.

Qin Q, Li Z, Zhang M, Dai Y, Li S, Wu H . Effects of melittin on production performance, antioxidant function, immune function, heat shock protein, intestinal morphology, and cecal microbiota in heat-stressed quails. Poult Sci. 2023; 102(10):102713. PMC: 10407909. DOI: 10.1016/j.psj.2023.102713. View

16.

Zhao L, Feng Y, Xu Z, Zhang N, Zhang W, Zuo G . Selenium mitigated aflatoxin B1-induced cardiotoxicity with potential regulation of 4 selenoproteins and ferroptosis signaling in chicks. Food Chem Toxicol. 2021; 154:112320. DOI: 10.1016/j.fct.2021.112320. View

17.

Ahmed B, Younas U, Asar T, Monteiro A, Hayen M, Tao S . Maternal heat stress reduces body and organ growth in calves: Relationship to immune status. JDS Commun. 2022; 2(5):295-299. PMC: 9623671. DOI: 10.3168/jdsc.2021-0098. View

18.

Aderao G, Jadhav S, Pattanaik A, Gupta S, Ramakrishnan S, Lokesha E . Dietary selenium levels modulates antioxidant, cytokine and immune response and selenoproteins mRNA expression in rats under heat stress condition. J Trace Elem Med Biol. 2022; 75:127105. DOI: 10.1016/j.jtemb.2022.127105. View

19.

Xiao X, Song D, Cheng Y, Hu Y, Wang F, Lu Z . Biogenic nanoselenium particles activate Nrf2-ARE pathway by phosphorylating p38, ERK1/2, and AKT on IPEC-J2 cells. J Cell Physiol. 2018; 234(7):11227-11234. DOI: 10.1002/jcp.27773. View

20.

Wu H, Luan Y, Wang H, Zhang P, Liu S, Wang P . Selenium inhibits ferroptosis and ameliorates autistic-like behaviors of BTBR mice by regulating the Nrf2/GPx4 pathway. Brain Res Bull. 2022; 183:38-48. DOI: 10.1016/j.brainresbull.2022.02.018. View