The Halophilic Bacterium for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources

Overview

Journal J Microbiol Biotechnol

Specialties Biotechnology
Microbiology

Date 2023 Nov 24

PMID 37997264

Authors

Seon Min Kim

Hye In Lee

Seung Won Nam

Deok Hyeon Jin

Gwi-Taek Jeong

Soo-Wan Nam

Brendan Burns

Young Jae Jeon

Affiliations

Soon will be listed here.

Abstract

The study objective was to evaluate the potential production of polyhydroxyalkanoates (PHAs), a biodegradable plastic material, by for which PHA production has never been reported. To identify the most effective nitrogen-limited culture conditions for PHAs production from this bacterium, batch fermentation using glucose concentrations ranging from 4 g l to 20 g l with a fixed ammonium concentration of 0.5 g l was carried out at 30°C and pH 8.0. A glucose supplement of 12 g l produced the highest PHA concentration (1.6 g l) and PHA content (0.63 g g) thereby identifying the optimal condition for PHA production from this bacterium. Gas chromatography-mass spectrometry analysis suggests that mostly produced copolymer types of poly(3-hydroxybutyrate--3-hydroxyvalerate) [P(3HB--3HV)] from glucose concentrations at 12 g l or higher under the nitrogen-limited conditions. When several other single carbon sources were evaluated for the most efficient PHA production, fructose provided the highest biomass (2.8 g l), and PHAs (1.29 g l) concentrations. Results indicated that this bacterium mostly produced the copolymers P(3HB--3HV) from single carbon sources composing a range of 93-98% of 3-hydroxybutyrate and 2-7% of 3-hydroxyvalerate, whereas mannose-supplemented conditions produced the only homopolymer type of P(3HB). However, when propionic acid as a secondary carbon source were supplemented into the media, produced the copolymer P(3HB--3HV), composed of a 50% maximum monomeric unit of 3-hydroxyvaleric acid (3HV). However, as the concentration of propionic acid increased, cell biomass and PHAs concentrations substantially decreased due to cell toxicity.

References

Catalan A, Malan A, Ferreira F, Gill P, Batista S . Propionic acid metabolism and poly-3-hydroxybutyrate-co-3-hydroxyvalerate production by a prpC mutant of Herbaspirillum seropedicae Z69. J Biotechnol. 2018; 286:36-44. DOI: 10.1016/j.jbiotec.2018.09.008. View

Takahashi R, Castilho N, da Silva M, Miotto M, Lima A . Prospecting for Marine Bacteria for Polyhydroxyalkanoate Production on Low-Cost Substrates. Bioengineering (Basel). 2017; 4(3). PMC: 5615306. DOI: 10.3390/bioengineering4030060. View

Suriyamongkol P, Weselake R, Narine S, Moloney M, Shah S . Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants - a review. Biotechnol Adv. 2007; 25(2):148-75. DOI: 10.1016/j.biotechadv.2006.11.007. View

Hong K, Chen G, Yu P, Zhang G, Liu Y, Chua H . Effect of C:N molar ratio on monomer composition of polyhydroxyalkanoates produced by Pseudomonas mendocina 0806 and Pseudomonas pseudoalkaligenus YS1. Appl Biochem Biotechnol. 2000; 84-86:971-80. DOI: 10.1385/abab:84-86:1-9:971. View

Mitra R, Xu T, Xiang H, Han J . Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory. Microb Cell Fact. 2020; 19(1):86. PMC: 7137286. DOI: 10.1186/s12934-020-01342-z. View

Zheng Y, Chen J, Ma Y, Chen G . Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction. Metab Eng. 2019; 58:82-93. DOI: 10.1016/j.ymben.2019.07.004. View

Kanehisa M, Sato Y, Kawashima M, Furumichi M, Tanabe M . KEGG as a reference resource for gene and protein annotation. Nucleic Acids Res. 2015; 44(D1):D457-62. PMC: 4702792. DOI: 10.1093/nar/gkv1070. View

Anjum A, Zuber M, Zia K, Noreen A, Anjum M, Tabasum S . Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements. Int J Biol Macromol. 2016; 89:161-74. DOI: 10.1016/j.ijbiomac.2016.04.069. View

Khomlaem C, Aloui H, Oh W, Kim B . High cell density culture of Paracoccus sp. LL1 in membrane bioreactor for enhanced co-production of polyhydroxyalkanoates and astaxanthin. Int J Biol Macromol. 2021; 192:289-297. DOI: 10.1016/j.ijbiomac.2021.09.180. View

10.

Spurr A . A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969; 26(1):31-43. DOI: 10.1016/s0022-5320(69)90033-1. View

11.

Tan D, Wu Q, Chen J, Chen G . Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates. Metab Eng. 2014; 26:34-47. DOI: 10.1016/j.ymben.2014.09.001. View

12.

Saratale G, Oh M . Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock. Int J Biol Macromol. 2015; 80:627-35. DOI: 10.1016/j.ijbiomac.2015.07.034. View

13.

Geyer R, Jambeck J, Law K . Production, use, and fate of all plastics ever made. Sci Adv. 2017; 3(7):e1700782. PMC: 5517107. DOI: 10.1126/sciadv.1700782. View

14.

Quillaguaman J, Guzman H, Van-Thuoc D, Hatti-Kaul R . Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects. Appl Microbiol Biotechnol. 2009; 85(6):1687-96. DOI: 10.1007/s00253-009-2397-6. View

15.

Azizi N, Najafpour G, Younesi H . Acid pretreatment and enzymatic saccharification of brown seaweed for polyhydroxybutyrate (PHB) production using Cupriavidus necator. Int J Biol Macromol. 2017; 101:1029-1040. DOI: 10.1016/j.ijbiomac.2017.03.184. View

16.

Lee J, Kim Y, Choi T, Lee W, Kim Y . Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol. 2004; 54(Pt 5):1699-1702. DOI: 10.1099/ijs.0.63146-0. View

17.

Ueda S, Matsumoto S, Takagi A, Yamane T . Synthesis of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) from Methanol and n-Amyl Alcohol by the Methylotrophic Bacteria Paracoccus denitrificans and Methylobacterium extorquens. Appl Environ Microbiol. 1992; 58(11):3574-9. PMC: 183146. DOI: 10.1128/aem.58.11.3574-3579.1992. View

18.

Kumar P, Jun H, Kim B . Co-production of polyhydroxyalkanoates and carotenoids through bioconversion of glycerol by Paracoccus sp. strain LL1. Int J Biol Macromol. 2017; 107(Pt B):2552-2558. DOI: 10.1016/j.ijbiomac.2017.10.147. View

19.

Eronen-Rasimus E, Hultman J, Hai T, Pessi I, Collins E, Wright S . Sea-Ice Bacteria sp. Strain 363 and sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature. Appl Environ Microbiol. 2021; 87(17):e0092921. PMC: 8357295. DOI: 10.1128/AEM.00929-21. View

20.

Sarada R, Vidhyavathi R, Usha D, Ravishankar G . An efficient method for extraction of astaxanthin from green alga Haematococcus pluvialis. J Agric Food Chem. 2006; 54(20):7585-8. DOI: 10.1021/jf060737t. View