Cloning, Purification, and Biophysical Characterization of FemB Protein from Methicillin-Resistant Staphylococcus Aureus and Inhibitors Screening

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2023 Nov 22

PMID 37991634

Authors

Anjini Gayatri Akkiraju

Krishnam Raju Atcha

Someswar Rao Sagurthi

Affiliations

Soon will be listed here.

Abstract

Methicillin-resistant Staphylococcus aureus has emerged as a leading cause of nosocomial, community acquired infections worldwide. Earlier investigations revealed that mecA-encoded FEM proteins play a role in antimicrobial resistance by developing unique peptidoglycan cross-linking which helps in the formation of protective cell membrane. In view to this, present study focused on expression, purification FEM proteins, and FemB biophysical characterization with the aid of in silico and in vitro approaches. Furthermore, we carried out biological screening assays and identified the novel potent 1,2,3-triazole conjugated 1,3,4-oxadiazole hybrid molecule which could inhibit the MRSA than the proven oxacillin.

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