Detection of and Characterisation by Multiple-locus Variable-number Tandem Repeat Analysis in Bovine Bulk Tank Milk Samples

Overview

Journal Vet Med (Praha)

Specialty Veterinary Medicine

Date 2023 Nov 20

PMID 37982024

Authors

Berna Yanmaz

Ediz Kagan Ozgen

Affiliations

Soon will be listed here.

Abstract

is the aetiological agent of Q fever, which is highly prevalent in Turkiye, but information on the genetic profiles of the bacterium is limited. This study aimed to investigate the presence of in bovine bulk tank milk (BTM) samples by polymerase chain reaction (PCR) and to investigate the genotypes by means of multiple-locus variable-number tandem repeat analysis (MLVA). A total of 25 markets that sold raw cow's milk were analysed by conventional PCR analysis. An MLVA analysis was performed at six loci, namely MS23, MS24, MS27, MS28, MS33, and MS34, to determine the genotypic variations of found in the positive DNA samples. The DNA of was detected in 16% of the BTM samples. The strains identified in the bovine milk samples collected in this study were found to belong to the same genotypic group as those detected in the bovine milk samples gathered in Greece. As a result, both the presence and genotyping studies of on the BTM samples in Turkiye will contribute to the determination of the geographical distribution of the agent.

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