Measuring and Modeling Forces Generated by Microtubules

Overview

Journal Biophys Rev

Publisher Springer

Specialty Biophysics

Date 2023 Nov 17

PMID 37974983

Authors

Nikita B Gudimchuk

Veronika V Alexandrova

Affiliations

Soon will be listed here.

Abstract

Tubulins are essential proteins, which are conserved across all eukaryotic species. They polymerize to form microtubules, cytoskeletal components of paramount importance for cellular mechanics. The microtubules combine an extraordinarily high flexural rigidity and a non-equilibrium behavior, manifested in their intermittent assembly and disassembly. These chemically fueled dynamics allow microtubules to generate significant pushing and pulling forces at their ends to reposition intracellular organelles, remodel membranes, bear compressive forces, and transport chromosomes during cell division. In this article, we review classical and recent studies, which have allowed the quantification of microtubule-generated forces. The measurements, to which we owe most of the quantitative information about microtubule forces, were carried out in biochemically reconstituted systems We also discuss how mathematical and computational modeling has contributed to the interpretations of these results and shaped our understanding of the mechanisms of force production by tubulin polymerization and depolymerization.

Citing Articles

VII Congress of Russian Biophysicists-2023, Krasnodar, Russia.

Anashkina A, Rubin A, Gudimchuk N, Vanin A, Tsygankov A, Orlov Y Biophys Rev. 2023; 15(5):801-805.

PMID: 37975012 PMC: 10643460. DOI: 10.1007/s12551-023-01164-4.

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