GmWRKY17-mediated Transcriptional Regulation of GmDREB1D and GmABA2 Controls Drought Tolerance in Soybean

Overview

Journal Plant Mol Biol

Date 2023 Nov 16

PMID 37973764

Authors

Yi Liu

Yueping Cao

Affiliations

Soon will be listed here.

Abstract

Drought affects soybean growth and ultimately led to yield reduction. WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2, and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway.

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References

Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K . Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell. 2003; 15(1):63-78. PMC: 143451. DOI: 10.1105/tpc.006130. View

Boudsocq M, Barbier-Brygoo H, Lauriere C . Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem. 2004; 279(40):41758-66. DOI: 10.1074/jbc.M405259200. View

Boyer J . Plant productivity and environment. Science. 1982; 218(4571):443-8. DOI: 10.1126/science.218.4571.443. View

Chen W, Yao Q, Patil G, Agarwal G, Deshmukh R, Lin L . Identification and Comparative Analysis of Differential Gene Expression in Soybean Leaf Tissue under Drought and Flooding Stress Revealed by RNA-Seq. Front Plant Sci. 2016; 7:1044. PMC: 4950259. DOI: 10.3389/fpls.2016.01044. View

Chen J, Nolan T, Ye H, Zhang M, Tong H, Xin P . Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell. 2017; 29(6):1425-1439. PMC: 5502465. DOI: 10.1105/tpc.17.00364. View

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Eulgem T, Rushton P, Schmelzer E, Hahlbrock K, Somssich I . Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J. 1999; 18(17):4689-99. PMC: 1171542. DOI: 10.1093/emboj/18.17.4689. View

Guttikonda S, Valliyodan B, Neelakandan A, Tran L, Kumar R, Quach T . Overexpression of AtDREB1D transcription factor improves drought tolerance in soybean. Mol Biol Rep. 2014; 41(12):7995-8008. DOI: 10.1007/s11033-014-3695-3. View

Haake V, Cook D, Riechmann J, Pineda O, Thomashow M, Zhang J . Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol. 2002; 130(2):639-48. PMC: 166593. DOI: 10.1104/pp.006478. View

10.

Hao Y, Wei W, Song Q, Chen H, Zhang Y, Wang F . Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants. Plant J. 2011; 68(2):302-13. DOI: 10.1111/j.1365-313X.2011.04687.x. View

11.

Huang K, Wu T, Ma Z, Li Z, Chen H, Zhang M . Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth. Int J Mol Sci. 2021; 22(9). PMC: 8122443. DOI: 10.3390/ijms22094337. View

12.

Ishihama N, Yamada R, Yoshioka M, Katou S, Yoshioka H . Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Plant Cell. 2011; 23(3):1153-70. PMC: 3082260. DOI: 10.1105/tpc.110.081794. View

13.

Jiang Y, Liang G, Yu D . Activated expression of WRKY57 confers drought tolerance in Arabidopsis. Mol Plant. 2012; 5(6):1375-88. DOI: 10.1093/mp/sss080. View

14.

Jiang J, Ma S, Ye N, Jiang M, Cao J, Zhang J . WRKY transcription factors in plant responses to stresses. J Integr Plant Biol. 2016; 59(2):86-101. DOI: 10.1111/jipb.12513. View

15.

Joshi R, Wani S, Singh B, Bohra A, Dar Z, Lone A . Transcription Factors and Plants Response to Drought Stress: Current Understanding and Future Directions. Front Plant Sci. 2016; 7:1029. PMC: 4943945. DOI: 10.3389/fpls.2016.01029. View

16.

Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M . KEGG for linking genomes to life and the environment. Nucleic Acids Res. 2007; 36(Database issue):D480-4. PMC: 2238879. DOI: 10.1093/nar/gkm882. View

17.

Lim C, Kang K, Shim Y, Yoo S, Paek N . Inactivating transcription factor OsWRKY5 enhances drought tolerance through abscisic acid signaling pathways. Plant Physiol. 2021; 188(4):1900-1916. PMC: 8968288. DOI: 10.1093/plphys/kiab492. View

18.

Lin P, Hwang S, Endo A, Okamoto M, Koshiba T, Cheng W . Ectopic expression of ABSCISIC ACID 2/GLUCOSE INSENSITIVE 1 in Arabidopsis promotes seed dormancy and stress tolerance. Plant Physiol. 2006; 143(2):745-58. PMC: 1803738. DOI: 10.1104/pp.106.084103. View

19.

Nambara E, Marion-Poll A . Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol. 2005; 56:165-85. DOI: 10.1146/annurev.arplant.56.032604.144046. View

20.

Nilson S, Assmann S . The control of transpiration. Insights from Arabidopsis. Plant Physiol. 2007; 143(1):19-27. PMC: 1761994. DOI: 10.1104/pp.106.093161. View