The Region of Phage T4 Genes 34, 33 and 59: Primary Structures and Organization on the Genome

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Dec 9

PMID 3797242

Citations 11

Authors

S Hahn

U Kruse

W Ruger

Affiliations

Soon will be listed here.

Abstract

The product of gene 33 is essential for the regulation of late transcription and gene product 59 is required in recombination, DNA repair and replication. The exact functions of both proteins are not known. Restriction fragments spanning the genomic area of genes 33 and 59 have been cloned into phage M13 and a 4.9 kb nucleotide sequence has been determined. Translation of the DNA sequence predicted that gp33 contains 112 amino acids with a mol.wt. of 12.816 kd while gp59 is composed of 217 amino acids adding up to a mol.wt. of 25.967 kd. The genomic area studied here also contains 3 open reading frames of genes not identified to date and it is thought to include the NH2-terminal part of g34. One of the open reading frames seems to code for the 10 kd protein, probably involved in the regulation of transcription of bacteriophage T4. This protein is predicted to consist of 89 amino acid residues with a mol.wt. of 10.376 kd. Gene 33 and the gene for the 10 kd protein were cloned separately on high expression vectors resulting in over-production of the two proteins.

Citing Articles

Bacteriophage T4 genome.

Miller E, Kutter E, Mosig G, Arisaka F, Kunisawa T, Ruger W Microbiol Mol Biol Rev. 2003; 67(1):86-156, table of contents.

PMID: 12626685 PMC: 150520. DOI: 10.1128/MMBR.67.1.86-156.2003.

Either bacteriophage T4 RNase H or Escherichia coli DNA polymerase I is essential for phage replication.

Hobbs L, Nossal N J Bacteriol. 1996; 178(23):6772-7.

PMID: 8955295 PMC: 178574. DOI: 10.1128/jb.178.23.6772-6777.1996.

The asiA gene of bacteriophage T4 codes for the anti-sigma 70 protein.

Orsini G, Ouhammouch M, Le Caer J, Brody E J Bacteriol. 1993; 175(1):85-93.

PMID: 8416914 PMC: 196100. DOI: 10.1128/jb.175.1.85-93.1993.

Analysis of five presumptive protein-coding sequences clustered between the primosome genes, 41 and 61, of bacteriophages T4, T2, and T6.

Selick H, Stormo G, DYSON R, Alberts B J Virol. 1993; 67(4):2305-16.

PMID: 8383243 PMC: 240378. DOI: 10.1128/JVI.67.4.2305-2316.1993.

Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 protein.

Winkelman J, Kassavetis G, Geiduschek E J Bacteriol. 1994; 176(4):1164-71.

PMID: 8106327 PMC: 205169. DOI: 10.1128/jb.176.4.1164-1171.1994.

References

Shine J, Dalgarno L . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974; 71(4):1342-6. PMC: 388224. DOI: 10.1073/pnas.71.4.1342. View

Gribskov M, Burgess R . Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res. 1986; 14(16):6745-63. PMC: 311678. DOI: 10.1093/nar/14.16.6745. View

Wu R, Wu J, Yeh Y . Role of gene 59 of bacteriophage T4 in repair of UV-irradiated and alkylated DNA in vivo. J Virol. 1975; 16(1):5-16. PMC: 354626. DOI: 10.1128/JVI.16.1.5-16.1975. View

Stevens A, Rhoton J . Characterization of an inhibitor causing potassium chloride sensitivity of an RNA polymerase from T4 phage-infected Escherichia coli. Biochemistry. 1975; 14(23):5074-9. DOI: 10.1021/bi00694a007. View

Shah D . Replication and recombination of gene 59 mutant of bacteriophage T4D. J Virol. 1975; 17(1):175-82. PMC: 515401. DOI: 10.1128/JVI.17.1.175-182.1976. View

Walz A, Pirrotta V, Ineichen K . Lambda repressor regulates the switch between PR and Prm promoters. Nature. 1976; 262(5570):665-9. DOI: 10.1038/262665a0. View

Robson B, Suzuki E . Conformational properties of amino acid residues in globular proteins. J Mol Biol. 1976; 107(3):327-56. DOI: 10.1016/s0022-2836(76)80008-3. View

Wood W, Revel H . The genome of bacteriophage T4. Bacteriol Rev. 1976; 40(4):847-68. PMC: 413987. DOI: 10.1128/br.40.4.847-868.1976. View

Mattson T, Van Houwe G, BOLLE A, SELZER G, Epstein R . Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mol Gen Genet. 1977; 154(3):319-26. DOI: 10.1007/BF00571289. View

10.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

11.

Ruger W . Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides. Eur J Biochem. 1978; 88(1):109-17. DOI: 10.1111/j.1432-1033.1978.tb12427.x. View

12.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

13.

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

14.

Fukada K, Abelson J . DNA sequence of a T4 transfer RNA gene cluster. J Mol Biol. 1980; 139(3):377-91. DOI: 10.1016/0022-2836(80)90136-9. View

15.

DeLange R, Williams L, Searcy D . A histone-like protein (HTa) from Thermoplasma acidophilum. II. Complete amino acid sequence. J Biol Chem. 1981; 256(2):905-11. View

16.

Zuker M, Stiegler P . Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res. 1981; 9(1):133-48. PMC: 326673. DOI: 10.1093/nar/9.1.133. View

17.

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View

18.

Burton Z, Burgess R, Lin J, Moore D, Holder S, Gross C . The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12. Nucleic Acids Res. 1981; 9(12):2889-903. PMC: 326900. DOI: 10.1093/nar/9.12.2889. View

19.

Mickelson C, Wiberg J . Membrane-associated DNase activity controlled by genes 46 and 47 of bacteriophage T4D and elevated DNase activity associated with the T4 das mutation. J Virol. 1981; 40(1):65-77. PMC: 256596. DOI: 10.1128/JVI.40.1.65-77.1981. View

20.

Gold L, Pribnow D, Schneider T, Shinedling S, Singer B, Stormo G . Translational initiation in prokaryotes. Annu Rev Microbiol. 1981; 35:365-403. DOI: 10.1146/annurev.mi.35.100181.002053. View