Genome Editing for Healthy Crops: Traits, Tools and Impacts

Overview

Journal Front Plant Sci

Date 2023 Nov 15

PMID 37965029

Authors

Kubilay Yildirim

Dragana Miladinovic

Jeremy Sweet

Meleksen Akin

Vladislava Galovic

Musa Kavas

Milica Zlatkovic

Eugenia de Andrade

Affiliations

Soon will be listed here.

Abstract

Crop cultivars in commercial use have often been selected because they show high levels of resistance to pathogens. However, widespread cultivation of these crops for many years in the environments favorable to a pathogen requires durable forms of resistance to maintain "healthy crops". Breeding of new varieties tolerant/resistant to biotic stresses by incorporating genetic components related to durable resistance, developing new breeding methods and new active molecules, and improving the Integrated Pest Management strategies have been of great value, but their effectiveness is being challenged by the newly emerging diseases and the rapid change of pathogens due to climatic changes. Genome editing has provided new tools and methods to characterize defense-related genes in crops and improve crop resilience to disease pathogens providing improved food security and future sustainable agricultural systems. In this review, we discuss the principal traits, tools and impacts of utilizing genome editing techniques for achieving of durable resilience and a "healthy plants" concept.

Citing Articles

Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering.

Lim S, Lee S J Agric Food Chem. 2024; 72(21):11871-11884.

PMID: 38744727 PMC: 11141556. DOI: 10.1021/acs.jafc.4c01650.

References

Chadwick M, Trewin H, Gawthrop F, Wagstaff C . Sesquiterpenoids lactones: benefits to plants and people. Int J Mol Sci. 2013; 14(6):12780-805. PMC: 3709812. DOI: 10.3390/ijms140612780. View

Luo D, Huguet-Tapia J, Raborn R, White F, Brendel V, Yang B . The resistance gene guards the rice susceptibility gene against exploitation by the bacterial blight pathogen. Plant Commun. 2021; 2(3):100164. PMC: 8132128. DOI: 10.1016/j.xplc.2021.100164. View

Yu Y, Gui Y, Li Z, Jiang C, Guo J, Niu D . Induced Systemic Resistance for Improving Plant Immunity by Beneficial Microbes. Plants (Basel). 2022; 11(3). PMC: 8839143. DOI: 10.3390/plants11030386. View

Saijo Y, Loo E, Yasuda S . Pattern recognition receptors and signaling in plant-microbe interactions. Plant J. 2017; 93(4):592-613. DOI: 10.1111/tpj.13808. View

Malnoy M, Viola R, Jung M, Koo O, Kim S, Kim J . DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins. Front Plant Sci. 2017; 7:1904. PMC: 5170842. DOI: 10.3389/fpls.2016.01904. View

Piatek A, Ali Z, Baazim H, Li L, Abulfaraj A, Al-Shareef S . RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors. Plant Biotechnol J. 2014; 13(4):578-89. DOI: 10.1111/pbi.12284. View

Yang Y, Ahammed G, Wu C, Fan S, Zhou Y . Crosstalk among Jasmonate, Salicylate and Ethylene Signaling Pathways in Plant Disease and Immune Responses. Curr Protein Pept Sci. 2015; 16(5):450-61. DOI: 10.2174/1389203716666150330141638. View

Lykogianni M, Bempelou E, Karamaouna F, Aliferis K . Do pesticides promote or hinder sustainability in agriculture? The challenge of sustainable use of pesticides in modern agriculture. Sci Total Environ. 2021; 795:148625. DOI: 10.1016/j.scitotenv.2021.148625. View

Zafar K, Khan M, Amin I, Mukhtar Z, Yasmin S, Arif M . Precise CRISPR-Cas9 Mediated Genome Editing in Super Basmati Rice for Resistance Against Bacterial Blight by Targeting the Major Susceptibility Gene. Front Plant Sci. 2020; 11:575. PMC: 7304078. DOI: 10.3389/fpls.2020.00575. View

10.

Aman R, Ali Z, Butt H, Mahas A, Aljedaani F, Khan M . RNA virus interference via CRISPR/Cas13a system in plants. Genome Biol. 2018; 19(1):1. PMC: 5755456. DOI: 10.1186/s13059-017-1381-1. View

11.

Andersen E, Ali S, Byamukama E, Yen Y, Nepal M . Disease Resistance Mechanisms in Plants. Genes (Basel). 2018; 9(7). PMC: 6071103. DOI: 10.3390/genes9070339. View

12.

Gomez M, Lin Z, Moll T, Chauhan R, Hayden L, Renninger K . Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence. Plant Biotechnol J. 2018; 17(2):421-434. PMC: 6335076. DOI: 10.1111/pbi.12987. View

13.

Ji X, Zhang H, Zhang Y, Wang Y, Gao C . Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants. Nat Plants. 2016; 1:15144. DOI: 10.1038/nplants.2015.144. View

14.

Jia H, Zhang Y, Orbovic V, Xu J, White F, Jones J . Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker. Plant Biotechnol J. 2016; 15(7):817-823. PMC: 5466436. DOI: 10.1111/pbi.12677. View

15.

Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X . CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Nat Methods. 2017; 14(7):710-712. DOI: 10.1038/nmeth.4327. View

16.

Secgin Z, Uluisik S, Yildirim K, Abdulla M, Mostafa K, Kavas M . Genome-Wide Identification of the Aconitase Gene Family in Tomato () and CRISPR-Based Functional Characterization of on Male-Sterility. Int J Mol Sci. 2022; 23(22). PMC: 9699144. DOI: 10.3390/ijms232213963. View

17.

Butt H, Jamil M, Wang J, Al-Babili S, Mahfouz M . Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis. BMC Plant Biol. 2018; 18(1):174. PMC: 6116466. DOI: 10.1186/s12870-018-1387-1. View

18.

Bartlett M, Moyers B, Man J, Subramaniam B, Makunga N . The Power and Perils of De Novo Domestication Using Genome Editing. Annu Rev Plant Biol. 2022; 74:727-750. DOI: 10.1146/annurev-arplant-053122-030653. View

19.

Thomma B, Nurnberger T, Joosten M . Of PAMPs and effectors: the blurred PTI-ETI dichotomy. Plant Cell. 2011; 23(1):4-15. PMC: 3051239. DOI: 10.1105/tpc.110.082602. View

20.

Boschi F, Schvartzman C, Murchio S, Ferreira V, Siri M, Galvan G . Enhanced Bacterial Wilt Resistance in Potato Through Expression of EFR and Introgression of Quantitative Resistance from . Front Plant Sci. 2017; 8:1642. PMC: 5627020. DOI: 10.3389/fpls.2017.01642. View