Association of Trophectoderm MRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Overview

Journal Reprod Sci

Publisher Springer

Specialty Reproductive Medicine

Date 2023 Nov 14

PMID 37964074

Authors

Masoumeh Esmaeilivand

Aliyeh Ghasemzadeh

Behrooz Niknafs

Amir Fattahi

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) and mRNAs can serve as indicators of the chromosomal state of an embryo, with different profiles observed in euploid and aneuploid blastocysts. Examining the levels of miRNAs associated with aneuploidy and euploidy, as well as mRNAs related to implantation, can aid in predicting blastocyst chromosomal normality and improving assisted reproductive technology (ART) outcomes. This study analyzed chromosomal abnormality of 25 blastocysts using fluorescence in situ hybridization (FISH) and also the expression of genes ERBB4, SELL, ITGB3, and ITGAV, as well as miRNAs, miR-339, miR-27b, miR-661, miR-30c, miR-191, miR-345, miR-142, miR-141, miR-20a, and miR-372. We found that 17 out of 25 embryos were aneuploid. Moreover, results revealed lower expression levels of miR-30c and miR-372 in aneuploid embryos compared to euploid ones, while ITGAV and ITGB3 showed significantly higher expression in aneuploid embryos. These findings suggest that miR-372, miR-30c, ITGAV, and ITGB3 expression in trophectoderm cells can serve as biomarkers for assessing embryo health.

Citing Articles

Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review.

Almutlaq A, Vinals Gonzalez X, SenGupta S Front Reprod Health. 2024; 6:1370341.

PMID: 38550247 PMC: 10973143. DOI: 10.3389/frph.2024.1370341.

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