Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveals a Differential Subcutaneous Adipose Tissue Mechanism Among Ningxiang Pig and Berkshires, and Their Offspring

Overview

Journal Animals (Basel)

Date 2023 Nov 14

PMID 37958077

Authors

Xiaoxiao Deng

Yuebo Zhang

Gang Song

Yawei Fu

Yue Chen

Hu Gao

Qian Wang

Zhao Jin

Yulong Yin

Kang Xu

Affiliations

Soon will be listed here.

Abstract

Adipose tissue composition contributes greatly to the quality and nutritional value of meat. Transcriptomic and lipidomic techniques were used to investigate the molecular mechanisms of the differences in fat deposition in Ningxiang pigs, Berkshires and F offspring. Transcriptomic analysis identified 680, 592, and 380 DEGs in comparisons of Ningxiang pigs vs. Berkshires, Berkshires vs. F offspring, and Ningxiang pigs vs. F offspring. The lipidomic analysis screened 423, 252, and 50 SCLs in comparisons of Ningxiang pigs vs. Berkshires, Berkshires vs. F offspring, and Ningxiang pigs vs. F offspring. Lycine, serine, and the threonine metabolism pathway, fatty acid biosynthesis and metabolism-related pathways were significantly enriched in comparisons of Berkshires vs. Ningxiang pigs and Berkshires vs. F offspring. The DEGs (, ) and the SCLs (phosphatidylserines) may have a great impact on the glycine, serine, and the threonine metabolism pathway. Moreover, the DEGs (, , , , , , , , , and ) and the SCLs (palmitoleic acid, linoleic acid, arachidonic acid, and icosadienoic acid) play important roles in the fatty acid biosynthesis and metabolism of fatty acids. Thus, the difference in fat deposition among Ningxiang pig, Berkshires, and F offspring may be caused by differences in the expression patterns of key genes in multiple enriched KEGG pathways. This research revealed multiple lipids that are potentially available biological indicators and screened key genes that are potential targets for molecular design breeding. The research also explored the molecular mechanisms of the difference in fat deposition among Ningxiang pig, Berkshires, and F pigs, and provided an insight into selection for backfat thickness and the fat composition of adipose tissue for future breeding strategies.

Citing Articles

Copy Number Variation and Selection Signal: Exploring the Domestication History and Phenotype Differences Between Duroc and the Chinese Native Ningxiang Pigs.

Yang F, Chen W, Yang Y, Meng Y, Chen Y, Ding X Int J Mol Sci. 2024; 25(21).

PMID: 39519268 PMC: 11546390. DOI: 10.3390/ijms252111716.

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