Superoxide Dismutase Gene Family in Cassava Revealed Their Involvement in Environmental Stress Via Genome-wide Analysis

Overview

Journal iScience

Publisher Cell Press

Date 2023 Nov 13

PMID 37954140

Authors

Linling Zheng

Abdoulaye Assane Hamidou

Xuerui Zhao

Zhiwei Ouyang

Hongxin Lin

Junyi Li

Xiaofei Zhang

Kai Luo

Yinhua Chen

Affiliations

Soon will be listed here.

Abstract

Superoxide dismutase (SOD) is a crucial metal-containing enzyme that plays a vital role in catalyzing the dismutation of superoxide anions, converting them into molecular oxygen and hydrogen peroxide, essential for enhancing plant stress tolerance. We identified 8 SOD genes (4 CSODs, 2 FSODs, and 2 MSODs) in cassava. Bioinformatics analyses provided insights into chromosomal location, phylogenetic relationships, gene structure, conserved motifs, and gene ontology annotations. genes were classified into two groups through phylogenetic analysis, revealing evolutionary connections. Promoters of these genes harbored stress-related -elements. Duplication analysis indicated the functional significance of MeCSOD2/MeCSOD4 and MeMSOD1/MeMSOD2. Through qRT-PCR, MeCSOD2 responded to salt stress, MeMSOD2 to drought, and cassava bacterial blight. Silencing MeMSOD2 increased CHN11 virulence, indicating MeMSOD2 is essential for cassava's defense against CHN11 infection. These findings enhance our understanding of the SOD gene family's role in cassava and contribute to strategies for stress tolerance improvement.

References

Ding Z, Fu L, Yan Y, Tie W, Xia Z, Wang W . Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava. PLoS One. 2017; 12(3):e0173043. PMC: 5332091. DOI: 10.1371/journal.pone.0173043. View

Bhatt I, Tripathi B . Plant peroxiredoxins: catalytic mechanisms, functional significance and future perspectives. Biotechnol Adv. 2011; 29(6):850-9. DOI: 10.1016/j.biotechadv.2011.07.002. View

Maruyama-Nakashita A, Nakamura Y, Watanabe-Takahashi A, Inoue E, Yamaya T, Takahashi H . Identification of a novel cis-acting element conferring sulfur deficiency response in Arabidopsis roots. Plant J. 2005; 42(3):305-14. DOI: 10.1111/j.1365-313X.2005.02363.x. View

Palma J, Mateos R, Lopez-Jaramillo J, Rodriguez-Ruiz M, Gonzalez-Gordo S, Lechuga-Sancho A . Plant catalases as NO and HS targets. Redox Biol. 2020; 34:101525. PMC: 7276441. DOI: 10.1016/j.redox.2020.101525. View

Diaz-Vivancos P, Faize M, Barba-Espin G, Faize L, Petri C, Hernandez J . Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums. Plant Biotechnol J. 2013; 11(8):976-85. DOI: 10.1111/pbi.12090. View

El-Sharkawy M . Cassava biology and physiology. Plant Mol Biol. 2005; 56(4):481-501. DOI: 10.1007/s11103-005-2270-7. View

Lopez C, Soto M, Restrepo S, Piegu B, Cooke R, Delseny M . Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray. Plant Mol Biol. 2005; 57(3):393-410. DOI: 10.1007/s11103-004-7819-3. View

McCord J, Fridovich I . Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969; 244(22):6049-55. View

Smith M, Doolittle R . A comparison of evolutionary rates of the two major kinds of superoxide dismutase. J Mol Evol. 1992; 34(2):175-84. DOI: 10.1007/BF00182394. View

10.

Osakabe Y, Yamaguchi-Shinozaki K, Shinozaki K, Tran L . ABA control of plant macroelement membrane transport systems in response to water deficit and high salinity. New Phytol. 2013; 202(1):35-49. DOI: 10.1111/nph.12613. View

11.

Chen Z, Scheffler B, Dennis E, Triplett B, Zhang T, Guo W . Toward sequencing cotton (Gossypium) genomes. Plant Physiol. 2007; 145(4):1303-10. PMC: 2151711. DOI: 10.1104/pp.107.107672. View

12.

Shiu S, Bleecker A . Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis. Plant Physiol. 2003; 132(2):530-43. PMC: 166995. DOI: 10.1104/pp.103.021964. View

13.

Jiang W, Yang L, He Y, Zhang H, Li W, Chen H . Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (). PeerJ. 2019; 7:e8062. PMC: 6873880. DOI: 10.7717/peerj.8062. View

14.

Papong S, Malakul P . Life-cycle energy and environmental analysis of bioethanol production from cassava in Thailand. Bioresour Technol. 2009; 101 Suppl 1:S112-8. DOI: 10.1016/j.biortech.2009.09.006. View

15.

Kliebenstein D, Monde R, Last R . Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization. Plant Physiol. 1998; 118(2):637-50. PMC: 34840. DOI: 10.1104/pp.118.2.637. View

16.

Gupta A, Heinen J, Holaday A, Burke J, Allen R . Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci U S A. 1993; 90(4):1629-33. PMC: 45928. DOI: 10.1073/pnas.90.4.1629. View

17.

Wuerges J, Lee J, Yim Y, Yim H, Kang S, Carugo K . Crystal structure of nickel-containing superoxide dismutase reveals another type of active site. Proc Natl Acad Sci U S A. 2004; 101(23):8569-74. PMC: 423235. DOI: 10.1073/pnas.0308514101. View

18.

Kayum M, Park J, Nath U, Saha G, Biswas M, Kim H . Genome-wide characterization and expression profiling of PDI family gene reveals function as abiotic and biotic stress tolerance in Chinese cabbage (Brassica rapa ssp. pekinensis). BMC Genomics. 2017; 18(1):885. PMC: 5691835. DOI: 10.1186/s12864-017-4277-2. View

19.

Yuan J, Amend A, Borkowski J, DEMARCO R, Bailey W, Liu Y . MULTICLUSTAL: a systematic method for surveying Clustal W alignment parameters. Bioinformatics. 2000; 15(10):862-3. DOI: 10.1093/bioinformatics/15.10.862. View

20.

Perry J, Shin D, Getzoff E, Tainer J . The structural biochemistry of the superoxide dismutases. Biochim Biophys Acta. 2009; 1804(2):245-62. PMC: 3098211. DOI: 10.1016/j.bbapap.2009.11.004. View