Preparation of Bacterial Cellulose-based Antibacterial Membranes with Prolonged Release of Drugs: Emphasis on the Chemical Structure of Drugs

Bacterial cellulose (BC) based antibacterial membranes were synthesized, including BC-cefoperazone (BC-CEF) and BC-cefoperazone sodium (BC-CEF/Na). To examine the various drug loading processes, the structure, morphology, and physical-chemical characteristics of membranes were evaluated. Results demonstrated that both types of medicines were successfully absorbed into membranes, and membranes displayed identical morphology and FT-IR peaks. BC-CEF showed lower crystalline of XRD, which was likely caused by the combination of carboxyl and hydroxyl. However, there were no drug peaks seen in the membranes, indicating no alteration of ribbon crystallization of BC. Two types of antibacterial membranes have significantly distinct drug-loading traits and drug-releasing profiles. The drug loading rate of CEF (46.4 mg/g) was significantly greater than CEF/Na (30.3 mg/g). The cumulative drug-releasing profiles showed that only BC-CEF continues to release drugs for a lengthy period up to 48 h and exhibited good antimicrobial activity against S. aureus and E. coli until 48 h. The cytotoxicity assay demonstrated the great biocompatibility of all membranes. Findings indicated that BC-CEF has the potential use as a prolonged biocide in the biomedical. The idea that BC membranes can naturally incorporate the carboxyl groups from antibiotics is also innovative and can be useful in developing of drug delivery systems.