Response of Human Neutrophils to Formyl-peptide Modified at the Terminal Amino and Carboxyl Groups

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 1986 Dec 1

PMID 3793205

Citations 3

Authors

S Spisani

T Cavalletti

R Gavioli

A Scatturin

G Vertuani

S Traniello

Affiliations

Soon will be listed here.

Abstract

Two analogs of chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine were examined for their capacity to activate several functions of human neutrophils. The C-terminus methyl ester derivative of the chemotactic peptide was found to possess strong biological activity and was able to induce levels of chemotaxis, enzyme secretion, and superoxide generation comparable to those observed with the same concentrations of N-formyl-L-methionyl-L-leucyl-L-phenylalanine. The analog containing a tert-butyloxycarbonyl group at the N-terminus, as well as the C-terminal methyl ester, was completely devoid of activity towards neutrophils. From these results, it appears that the free carboxyl group is not necessary for biological function. In contrast, the substituent at the N-terminus may play a critical role in the induction of the cellular response.

Citing Articles

Platelet-leukocyte interaction: activation of rabbit platelets by FMLP-stimulated neutrophils.

Coeffier E, Joseph D, Prevost M, Vargaftig B Br J Pharmacol. 1987; 92(2):393-406.

PMID: 3118996 PMC: 1853659. DOI: 10.1111/j.1476-5381.1987.tb11336.x.

Chemotactic response of human monocytes to pentapeptide analog derived from immunodeficiency virus protein gp 120.

Spisani S, Gavioli R, Giuliani A, Cavalletti T, Marastoni M, Balboni G Inflammation. 1990; 14(1):55-60.

PMID: 2323806 DOI: 10.1007/BF00914029.

Modulation of neutrophil functions by activated platelet release factors.

Spisani S, Giuliani A, Cavalletti T, Zaccarini M, Milani L, Gavioli R Inflammation. 1992; 16(2):147-58.

PMID: 1317360 DOI: 10.1007/BF00918954.

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