E2/ER Signaling Mediates the Meiotic Arrest of Goat Intrafollicular Oocytes Induced by Follicle-stimulating Hormone

Overview

Journal J Anim Sci

Date 2023 Nov 5

PMID 37925610

Authors

Sihai Lu

Yaju Tang

Ru Yao

Rui Xu

Hui Zhang

Jie Liu

Yan Gao

Qiang Wei

Xiaoe Zhao

Jianpeng Liu

Bin Han

Meng-Hao Pan

Baohua Ma

Affiliations

Soon will be listed here.

Abstract

The increased production of high-quality oocytes lies at the heart of the search to accelerate the reproduction of high-quality breeding livestock using assisted reproductive technology. Follicle-stimulating hormone (FSH) maintains the arrest of oocyte meiosis during early follicular development in vivo and promotes the synchronous maturation of nucleus and cytoplasm to improve oocyte quality. However, the mechanism by which FSH maintains meiotic arrest in oocytes is still not fully understood. Oocytes spontaneously resume meiosis once released from the arrested state. In this study, we isolated goat antral follicles with a diameter of 2.0-4.0 mm, cultured them in vitro either with or without added FSH, and finally collected the oocytes to observe their meiotic state. The results showed that FSH effectively inhibited the meiotic recovery of oocytes in follicles [4 h: control (n = 84) vs. with FSH (n = 86), P = .0115; 6 h: control (n = 86) vs. FSH (n = 85), P = 0.0308; and 8 h: control (n = 95) vs. FSH (n = 101), P = 0.0039]. FSH significantly inhibited the downregulation of natriuretic peptide receptor 2 (NPR2) expression and cyclic guanosine monophosphate (cGMP) synthesis during follicular culture in vitro (P < 0.05). Further exploration found that FSH promoted the synthesis of 17β-estradiol (E2) (P = .0249 at 4 h and P = .0039 at 8 h) and maintained the expression of the estrogen nuclear receptor ERβ, but not the estrogen nuclear receptor ERα during follicle culture in vitro (P = .0190 at 2 h, and P = .0100 at 4 h). In addition, E2/ER (estrogen nuclear receptors ERα and ERβ) mediated the inhibitory effect of FSH on the downregulation of NPR2 expression and cGMP synthesis, ultimately preventing the meiotic recovery of oocytes (P < .05). In summary, our study showed that FSH-induced estrogen production in goat follicles, and the E2/ER signaling pathway, both mediated meiotic arrest in FSH-induced goat oocytes.

Citing Articles

Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid.

Feugang J, Gad A, Menjivar N, Ishak G, Gebremedhn S, Gastal M J Anim Sci Biotechnol. 2024; 15(1):137.

PMID: 39380110 PMC: 11462823. DOI: 10.1186/s40104-024-01097-2.

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