Concentration-effect Relationship for Tranexamic Acid Inhibition of Tissue Plasminogen Activator-induced Fibrinolysis In vitro Using the Viscoelastic ClotPro® TPA-test

Overview

Journal Br J Anaesth

Publisher Elsevier

Specialty Anesthesiology

Date 2023 Nov 4

PMID 37925268

Authors

Christoph Dibiasi

Stefan Ulbing

Dagmar Bancher-Todesca

Martin Ulm

Johannes Gratz

Peter Quehenberger

Eva Schaden

Affiliations

Soon will be listed here.

Abstract

Background: Tranexamic acid is an antifibrinolytic drug that is commonly administered for obstetric haemorrhage. Conventional viscoelastic tests are not sensitive to tranexamic acid, but the novel ClotPro® TPA-test can measure tranexamic acid-induced inhibition of fibrinolysis. We aimed to evaluate the TPA-test in pregnant and non-pregnant women.

Methods: We performed an in vitro study of whole blood samples spiked with tranexamic acid from pregnant women in the first, second, and third trimester (n=20 per group) and from non-pregnant women (n=20). We performed ClotPro TPA-tests of whole blood sample and ClotPro EX-tests, FIB-tests, and TPA-tests.

Results: Clot lysis was inhibited in a concentration-dependent manner up to a tranexamic acid concentration of 6.25 mg L. At tranexamic acid concentrations of 12.5 mg L and above, clot lysis was completely inhibited. The concentration-effect relationship of tranexamic acid did not differ in a clinically important manner in blood from pregnant women across all three trimesters or from non-pregnant controls. A median maximum lysis cut-off value of at9 least 16% (25-75th percentiles 15-18), a median clot lysis time of 3600 s (25-75th percentiles 3600-3600), or both was associated with a tranexamic acid concentration of least 12.5 mg L.

Conclusions: The ClotPro® TPA-test is sensitive in detecting inhibition of fibrinolysis by tranexamic acid in whole blood samples of pregnant and non-pregnant women. The concentration-effect relationship of tranexamic acid to inhibit fibrinolysis in whole blood did not differ for women in the first, second, and third trimester or for non-pregnant women.

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