A Comprehensive Review on Genetic Modification of Plant Cell Wall for Improved Saccharification Efficiency

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2023 Nov 3

PMID 37921982

Authors

Abinash Mishra

Tapas Kumar Mishra

Spandan Nanda

Mahendra Kumar Mohanty

Manasi Dash

Affiliations

Soon will be listed here.

Abstract

The focus is now on harnessing energy from green sources through sustainable technology to minimize environmental pollution. Several crop residues including rice and wheat straw are having enormous potential to be used as lignocellulosic source material for bioenergy production. The lignocellulosic feedstock is primarily composed of cellulose, hemicellulose, and lignin cell wall polymers. The hemicellulose and lignin polymers induce crosslinks in the cell wall, by firmly associating with cellulose microfibrils, and thereby, denying considerable access of cellulose to cellulase enzymes. This issue has been addressed by various researchers through downregulating several genes associated in monolignol biosynthesis in Arabidopsis, Poplar, Rice and Switchgrass to increase ethanol recovery. Similarly, xylan biosynthetic genes are also targeted to genetically culminate its accumulation in the secondary cell walls. Regulation of cellulose synthases (CesA) proves to be an effective tool in addressing the negative impact of these two factors. Modification in the expression of cellulose synthase aids in reducing cellulose crystallinity as well as polymerisation degree which in turn increases ethanol recovery. The engineered bioenergy crops and various fungal strains with state of art biomass processing techniques presents the most recent integrative biotechnology model for cost effective green fuels generation along with production of key value-added products with minuscule disturbances in the environment. Plant breeding strategies utilizing the existing variability for biomass traits will be key in developing dual purpose varieties. For this purpose, reorientation of conventional breeding techniques for incorporating useful biomass traits will be effective.

Citing Articles

The complexity of kodo millet: genomic analysis and implications in crop improvement.

Mishra P, Nanda S, Barpanda T, Dash M, Dash S, Choudhury S Planta. 2024; 261(1):15.

PMID: 39680216 DOI: 10.1007/s00425-024-04588-8.

Enhancing rice ecological production: synergistic effects of wheat-straw decomposition and microbial agents on soil health and yield.

Wen Y, Ma Y, Wu Z, Yang Y, Yuan X, Chen K Front Plant Sci. 2024; 15:1368184.

PMID: 39175490 PMC: 11338901. DOI: 10.3389/fpls.2024.1368184.

Improvement of little millet (Panicum sumatrense) using novel omics platform and genetic resource integration.

Mishra A, Dash S, Barpanda T, Choudhury S, Mishra P, Dash M Planta. 2024; 260(3):60.

PMID: 39052093 DOI: 10.1007/s00425-024-04493-0.

A Comprehensive Molecular, Biochemical, Histochemical, and Spectroscopic Characterization of Early and Medium Duration Rice Genotypes Investigating Dry Matter Accumulation Efficiencies.

Mishra A, Dash M, Barpanda T, Sibadatta A, Sahu P, Sahu P Appl Biochem Biotechnol. 2024; 196(11):8117-8133.

PMID: 38687455 DOI: 10.1007/s12010-024-04950-2.

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