Sp. Nov., Isolated from Horse Faeces

Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25-45 °C (optimum, 35 °C); pH 6-9 (optimum, pH 6); and in the presence of 0-6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108 forms a phyletic lineage distinct from other closely related species within the genus . It was found to be most closely related to ATCC 25577 (98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108 was 63.2 mol%. The DNA-DNA hybridization values of strain CBA3108 with ATCC 25577, WCA-380-WT-3A and subsp. DSM 1897 were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is CBA3108 (=KACC 22889=JCM 35966).