In Ovo Vaccination with Recombinant Herpes Virus of the Turkey-Laryngotracheitis Vaccine Adjuvanted with CpG-Oligonucleotide Provides Protection Against a Viral Challenge in Broiler Chickens

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 Oct 28

PMID 37896880

Authors

Carissa Gaghan

Matthew Browning

Abdelhamid M Fares

Mohamed Faizal Abdul-Careem

Isabel M Gimeno

Raveendra R Kulkarni

Affiliations

Soon will be listed here.

Abstract

Infectious laryngotracheitis (ILT) is an economically important disease in chickens. We previously showed that an in ovo adjuvantation of recombinant herpesvirus of the turkey-Laryngotracheitis (rHVT-LT) vaccine with CpG-oligonucleotides (ODN) can boost vaccine-induced responses in one-day-old broiler chickens. Here, we evaluated the protective efficacy of in ovo administered rHVT-LT + CpG-ODN vaccination against a wild-type ILT virus (ILTV) challenge at 28 days of age and assessed splenic immune gene expression as well as cellular responses. A chicken-embryo-origin (CEO)-ILT vaccine administered in water at 14 days of age was also used as a comparative control for the protection assessment. The results showed that the rHVT-LT + CpG-ODN or the CEO vaccinations provided significant protection against the ILTV challenge and that the level of protection induced by both the vaccines was statistically similar. The protected birds had a significantly upregulated expression of interferon (IFN)γ or interleukin (IL)-12 cytokine genes. Furthermore, the chickens vaccinated with the rHVT-LT + CpG-ODN or CEO vaccine had a significantly higher frequency of γδ T cells and activated CD4+ or CD8+ T cells, compared to the unvaccinated-ILTV challenge control. Collectively, our findings suggest that CpG-ODN can be used as an effective adjuvant for rHVT-LT in ovo vaccination to induce protective immunity against ILT in broiler chickens.

Citing Articles

How to Break through the Bottlenecks of in Ovo Vaccination in Poultry Farming.

Li X, Liu X, Cui L, Liu Z, Zhang Y, Li H Vaccines (Basel). 2024; 12(1).

PMID: 38250861 PMC: 10821430. DOI: 10.3390/vaccines12010048.

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