Ameliorates Oxidative Stress in Nucleus Pulposus Cells Via Downregulating the TNF-α Signaling Pathway

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2023 Oct 28

PMID 37895953

Authors

Peng Zhang

Jiahui He

Yanchi Gan

Qi Shang

Honglin Chen

Wenhua Zhao

Gengyang Shen

Xiaobing Jiang

Hui Ren

Affiliations

Soon will be listed here.

Abstract

(PT), a widely used traditional Chinese medicine, exerts protective effects against bone diseases such as intervertebral disc degeneration (IDD). Despite its effectiveness, the molecular mechanisms underlying the effects of PT on IDD remain unclear. In this study, we used a comprehensive strategy combining bioinformatic analysis with experimental verification to investigate the possible molecular mechanisms of PT against IDD. We retrieved targets for PT and IDD, and then used their overlapped targets for protein-protein interaction (PPI) analysis. In addition, we used Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to investigate the anti-IDD mechanisms of PT. Moreover, in vivo and in vitro experiment validations including hematoxylin-eosin (HE) and safranine O-green staining, senescence-associated β-galactosidase (SA-β-gal) assay, cell immunofluorescence staining, intracellular ROS measurement and Western blot analysis were performed to verify bioinformatics findings. We identified 342 and 872 PT- and IDD-related targets (32 overlapping targets). GO enrichment analysis yielded 450 terms related to oxidative stress and inflammatory response regulation. KEGG analysis identified 48 signaling pathways, 10 of which were significant; the TNF-α signaling pathway had the highest -value, and prostaglandin G/H synthase 2 (), endothelin-1 (), TNF-α, and were enriched in this pathway. Histopathological results and safranin O/green staining demonstrated that PT attenuated IDD, and SA-β-gal assay showed that PT ameliorated nucleus pulposus cell (NPC) senescence. An ROS probe was adopted to confirm the protective effect of PT against oxidative stress. Western blot analyses confirmed that PT downregulated the protein expression of , , TNF-α, and in the TNF-α signaling pathway as well as cellular senescence marker p16, proinflammatory cytokine interleukin-6 (), while PT upregulated the expression of NPC-specific markers including and in a concentration-dependent manner. To the best of our knowledge, this study is the first to report that PT alleviates IDD by downregulating the protein expression of , , TNF-α, and in the TNF-α signaling pathway and upregulating that of and , thus suppressing inflammatory responses and oxidative stress in NPCs.

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