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Phylogenomic Analysis of Gene Superfamily and Their Association with Flavonoids Biosynthesis in Peanut ( L.)

Overview
Journal Genes (Basel)
Publisher MDPI
Date 2023 Oct 28
PMID 37895293
Authors
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Abstract

Cytochrome P450s (CYPs) constitute extensive enzyme superfamilies in the plants, playing pivotal roles in a multitude of biosynthetic and detoxification pathways essential for growth and development, such as the flavonoid biosynthesis pathway. However, CYPs have not yet been systematically studied in the cultivated peanuts ( L.), a globally significant cash crop. This study addresses this knowledge deficit through a comprehensive genome-wide analysis, leading to the identification of 589 genes in peanuts. Through phylogenetic analysis, all AhCYPs were systematically classified into 9 clans, 43 gene families. The variability in the number of gene family members suggests specialization in biological functions. Intriguingly, both tandem duplication and fragment duplication events have emerged as pivotal drivers in the evolutionary expansion of the superfamily. Ka/Ks analysis underscored the substantial influence of strong purifying selection on the evolution of . Furthermore, we selected 21 genes encoding 8 enzymes associated with the flavonoid pathway. The results of quantitative real-time PCR (qRT-PCR) experiments unveiled stage-specific expression patterns during the development of peanut testa, with discernible variations between pink and red testa. Importantly, we identified a direct correlation between gene expression levels and the accumulation of metabolites. These findings offer valuable insights into elucidating the comprehensive functions of and the underlying mechanisms governing the divergent accumulation of flavonoids in testa of different colors.

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References
1.
Williams P, Cosme J, Sridhar V, Johnson E, McRee D . Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversity. Mol Cell. 2000; 5(1):121-31. DOI: 10.1016/s1097-2765(00)80408-6. View

2.
Grotewold E . The genetics and biochemistry of floral pigments. Annu Rev Plant Biol. 2006; 57:761-80. DOI: 10.1146/annurev.arplant.57.032905.105248. View

3.
Paquette S, Bak S, Feyereisen R . Intron-exon organization and phylogeny in a large superfamily, the paralogous cytochrome P450 genes of Arabidopsis thaliana. DNA Cell Biol. 2000; 19(5):307-17. DOI: 10.1089/10445490050021221. View

4.
Zhang K, Ma J, Gangurde S, Hou L, Xia H, Li N . Targeted metabolome analysis reveals accumulation of metabolites in testa of four peanut germplasms. Front Plant Sci. 2022; 13:992124. PMC: 9523574. DOI: 10.3389/fpls.2022.992124. View

5.
Liu C, Huhman D, Sumner L, Dixon R . Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula. Plant J. 2003; 36(4):471-84. DOI: 10.1046/j.1365-313x.2003.01893.x. View