Genome-Wide Identification and Expression Analysis of BZIP Family Genes in

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Oct 28

PMID 37895267

Authors

Mengyang Wu

Jinsong Chen

Weilin Tang

Yijie Jiang

Zhaoyong Hu

Dongbei Xu

Kai Hou

Yinyin Chen

Wei Wu

Affiliations

Soon will be listed here.

Abstract

The basic (region) leucine zippers (bZIPs) are evolutionarily conserved transcription factors widely distributed in eukaryotic organisms. In plants, they are not only involved in growth and development, defense and stress responses and regulation of physiological processes but also play a pivotal role in regulating secondary metabolism. To explore the function related to the bZIP gene family in Bertoni, we identified 105 genes at the genome-wide level and classified them into 12 subfamilies using bioinformation methods. Three main classes of -acting elements were found in the promoter regions, including development-related elements, defense and stress-responsive elements and phytohormone-responsive elements. Through protein-protein interaction network of 105 SrbZIP proteins, SrbZIP proteins were mainly classified into four major categories: ABF2/ABF4/ABI5 (SrbZIP51/SrbZIP38/SrbZIP7), involved in phytohormone signaling, GBF1/GBF3/GBF4 (SrbZIP29/SrbZIP63/SrbZIP60) involved in environmental signaling, AREB3 (SrbZIP88), PAN (SrbZIP12), TGA1 (SrbZIP69), TGA4 (SrbZIP82), TGA7 (SrbZIP31), TGA9 (SrbZIP95), TGA10 (SrbZIP79) and HY5 (SrbZIP96) involved in cryptochrome signaling, and FD (SrbZIP72) promoted flowering. The transcriptomic data showed that genes were differentially expressed in six cultivars ('023', '110', 'B1188', '11-14', 'GP' and 'GX'). Moreover, the expression levels of selected 15 genes in response to light, abiotic stress (low temperature, salt and drought), phytohormones (methyl jasmonate, gibberellic acid and salicylic acid) treatment and in different tissues were analyzed utilizing qRT-PCR. Some genes were further identified to be highly induced by factors affecting glycoside synthesis. Among them, three genes (, and ) were predicted to be related to stress-responsive terpenoid synthesis in . The protein-protein interaction network expanded the potential functions of genes. This study firstly provided the comprehensive genome-wide report of the gene family, laying a foundation for further research on the evolution, function and regulatory role of the bZIP gene family in terpenoid synthesis in .

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