LSM14B is Essential for Oocyte Meiotic Maturation by Regulating Maternal MRNA Storage and Clearance

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2023 Oct 27

PMID 37889087

Authors

Yanling Wan

Shuang Yang

Tongtong Li

Yuling Cai

Xinyue Wu

Mingyu Zhang

Tahir Muhammad

Tao Huang

Yue Lv

Wai-Yee Chan

Gang Lu

Jingxin Li

Qian-Qian Sha

Zi-Jiang Chen

Hongbin Liu

Affiliations

Soon will be listed here.

Abstract

Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria-associated ribonucleoprotein domain (MARDO). However, the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4 and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, and couple the MARDO with mRNA clearance and oocyte meiotic maturation.

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