The Relationship Between Prognosis of Patients with Traumatic Brain Injury and MicroRNA Biogenesis Proteins

Overview

Journal Ulus Travma Acil Cerrahi Derg

Specialties Emergency Medicine
General Surgery

Date 2023 Oct 27

PMID 37889026

Authors

Ayse Cabukusta Acar

Sukran Burcak Yoldas

Elif Sarionder Gencer

Ilker Onguc Aycan

Suat Hayri Sanli

Affiliations

Soon will be listed here.

Abstract

Background: This study aims to investigate whether the expression levels of proteins involved in microRNA (miRNA) biogenesis vary in early- and late-stage traumatic brain injury (TBI) patients and to evaluate its effect on prognosis.

Methods: Dicer, Drosha, DiGeorge Syndrome Critical Region eight (DGCR8), Exportin5 (XPO5), and Argonaute2 (AGO2) levels were measured in the blood samples of severe TBI patients collected 4-6 h and 72 h after the trauma and compared with the control group. Prognostic follow-up of the patients was performed using the Glasgow Coma Scale score.

Results: There were no statistically significant changes in the expression of the miRNA biogenesis proteins Dicer, Drosha, DGCR8, XPO5, and AGO2 in patients with severe TBI. However, the expression of Dicer increased in the patients who improved from the severe TBI grade to the mild TBI grade, and the expression of AGO2 decreased in most of these patients. The Dicer expression profile was found to increase in patients discharged from the intensive care unit in a short time.

Conclusion: MicroRNAs and their biogenesis proteins may guide prognostic and therapeutic decisions for patients with TBI in the future.

References

Konopka W, Kiryk A, Novak M, Herwerth M, Parkitna J, Wawrzyniak M . MicroRNA loss enhances learning and memory in mice. J Neurosci. 2010; 30(44):14835-42. PMC: 6633640. DOI: 10.1523/JNEUROSCI.3030-10.2010. View

Rostami Mogaddam M, Ardabili N, Shafaeei Y, Maleki N, Jafari N, Jafari A . Overexpression of Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), and Dicer mRNAs in the pathogenesis of psoriasis. J Cosmet Dermatol. 2017; 16(4):e48-e53. DOI: 10.1111/jocd.12336. View

Wang W, Wilfred B, Madathil S, Tang G, Hu Y, Dimayuga J . miR-107 regulates granulin/progranulin with implications for traumatic brain injury and neurodegenerative disease. Am J Pathol. 2010; 177(1):334-45. PMC: 2893676. DOI: 10.2353/ajpath.2010.091202. View

Nallamshetty S, Chan S, Loscalzo J . Hypoxia: a master regulator of microRNA biogenesis and activity. Free Radic Biol Med. 2013; 64:20-30. PMC: 3762925. DOI: 10.1016/j.freeradbiomed.2013.05.022. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Chesnut R, Marshall L, Klauber M, Blunt B, Baldwin N, Eisenberg H . The role of secondary brain injury in determining outcome from severe head injury. J Trauma. 1993; 34(2):216-22. DOI: 10.1097/00005373-199302000-00006. View

Liu L, Sun T, Liu Z, Chen X, Zhao L, Qu G . Traumatic brain injury dysregulates microRNAs to modulate cell signaling in rat hippocampus. PLoS One. 2014; 9(8):e103948. PMC: 4121204. DOI: 10.1371/journal.pone.0103948. View

Patz S, Trattnig C, Grunbacher G, Ebner B, Gully C, Novak A . More than cell dust: microparticles isolated from cerebrospinal fluid of brain injured patients are messengers carrying mRNAs, miRNAs, and proteins. J Neurotrauma. 2013; 30(14):1232-42. DOI: 10.1089/neu.2012.2596. View

Cimmino A, Calin G, Fabbri M, Iorio M, Ferracin M, Shimizu M . miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci U S A. 2005; 102(39):13944-9. PMC: 1236577. DOI: 10.1073/pnas.0506654102. View

10.

Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C . Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014; 34(30):10055-71. PMC: 4107397. DOI: 10.1523/JNEUROSCI.1260-14.2014. View

11.

Bernstein E, Caudy A, Hammond S, Hannon G . Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 2001; 409(6818):363-6. DOI: 10.1038/35053110. View

12.

Finch C, McCrory P, Ewing M, Sullivan S . Concussion guidelines need to move from only expert content to also include implementation and dissemination strategies. Br J Sports Med. 2012; 47(1):12-4. DOI: 10.1136/bjsports-2012-091796. View

13.

Di Pietro V, Yakoub K, Scarpa U, Di Pietro C, Belli A . MicroRNA Signature of Traumatic Brain Injury: From the Biomarker Discovery to the Point-of-Care. Front Neurol. 2018; 9:429. PMC: 6010584. DOI: 10.3389/fneur.2018.00429. View

14.

Karp X, Ambros V . Developmental biology. Encountering microRNAs in cell fate signaling. Science. 2005; 310(5752):1288-9. DOI: 10.1126/science.1121566. View

15.

Fagerberg L, Hallstrom B, Oksvold P, Kampf C, Djureinovic D, Odeberg J . Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics. 2013; 13(2):397-406. PMC: 3916642. DOI: 10.1074/mcp.M113.035600. View

16.

Todaka H, Higuchi T, Yagyu K, Sugiyama Y, Yamaguchi F, Morisawa K . Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers. Mol Cell Biol. 2015; 35(13):2295-308. PMC: 4456438. DOI: 10.1128/MCB.01297-14. View

17.

Dharap A, Bowen K, Place R, Li L, Vemuganti R . Transient focal ischemia induces extensive temporal changes in rat cerebral microRNAome. J Cereb Blood Flow Metab. 2009; 29(4):675-87. PMC: 2743462. DOI: 10.1038/jcbfm.2008.157. View

18.

Mulligan M, Dubose C, Yue J, Miles M, Lu L, Hamre K . Expression, covariation, and genetic regulation of miRNA Biogenesis genes in brain supports their role in addiction, psychiatric disorders, and disease. Front Genet. 2013; 4:126. PMC: 3701868. DOI: 10.3389/fgene.2013.00126. View

19.

Kroemer G, Marino G, Levine B . Autophagy and the integrated stress response. Mol Cell. 2010; 40(2):280-93. PMC: 3127250. DOI: 10.1016/j.molcel.2010.09.023. View

20.

Murray G, Butcher I, McHugh G, Lu J, Mushkudiani N, Maas A . Multivariable prognostic analysis in traumatic brain injury: results from the IMPACT study. J Neurotrauma. 2007; 24(2):329-37. DOI: 10.1089/neu.2006.0035. View