A Simple Protocol for Isolating Hemolymph from Single Adult Flies

Overview

Journal Methods Protoc

Specialty General Medicine

Date 2023 Oct 27

PMID 37888032

Authors

Kirah Jones

Ioannis Eleftherianos

Affiliations

Soon will be listed here.

Abstract

is an excellent model for dissecting innate immune signaling and functions. Humoral and cellular immune mechanisms in the fly take place in the hemolymph, where host defense components are secreted and act in response to microbial invaders. Studying hemolymph factors is critical for understanding the regulation of the host's antimicrobial immune system. Therefore, methods for extracting the fly hemolymph efficiently and in sufficient quantities are essential for isolating and characterizing immune proteins and peptides. Here, we describe a novel and simple hemolymph isolation protocol for single male and female adults. This procedure substantially improves the already used technique and allows fly immunologists to explore innate immune hemolymph activity in individuals.

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