Deciphering the Chemical Fingerprint of : Volatile Components Attractive to Wasps

Overview

Journal Insects

Specialty Biology

Date 2023 Oct 27

PMID 37887821

Authors

Chaoran Zhang

Penghua Bai

Jie Kang

Tian Dong

Haixia Zheng

Xianhong Zhang

Affiliations

Soon will be listed here.

Abstract

is a pest that poses a serious threat to the yield and quality of seeds. In this study, we employed solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) to identify volatile organic compounds (VOCs) in pods during the pod-filled period. Additionally, we utilized a Y-tube olfactometer to measure the behavioral response of to different individual VOCs and specific VOC-based formulations. The most effective formulations were further evaluated for their effectiveness in attracting wasps in the field. Our findings revealed that pods emit 25 VOCs, including green leaf volatiles (GLVs) and terpenoid and aromatic compounds. Among these compounds, five were found to be most attractive to at the following concentrations: 10 µg/µL cis-β-ocimene, 500 µg/µL hexyl acetate, 100 µg/µL hexanal, 1 µg/µL decanal, and 10 µg/µL β-caryophyllene, with respective response rates of 67.65%, 67.74%, 65.12%, 67.57%, and 66.67%. In addition, we evaluated 26 mixed VOC formulations, and three of them were effective at attracting . Furthermore, field experiments showed that one of the formulations was significantly more effective than the others, which could be used for monitoring populations.

References

Song C, Ma L, Zhao J, Xue Z, Yan X, Hao C . Electrophysiological and Behavioral Responses of (Lepidoptera: Plutellidae) to Volatiles from a Non-host Plant, Geranium, (Geraniaceae). J Agric Food Chem. 2022; 70(20):5982-5992. DOI: 10.1021/acs.jafc.1c08165. View

Jing T, Du W, Gao T, Wu Y, Zhang N, Zhao M . Herbivore-induced DMNT catalyzed by CYP82D47 plays an important role in the induction of JA-dependent herbivore resistance of neighboring tea plants. Plant Cell Environ. 2020; 44(4):1178-1191. DOI: 10.1111/pce.13861. View

Ortiz-Carreon F, Rojas J, Cisneros J, Malo E . Herbivore-Induced Volatiles from Maize Plants Attract Chelonus insularis, an Egg-Larval Parasitoid of the Fall Armyworm. J Chem Ecol. 2019; 45(3):326-337. DOI: 10.1007/s10886-019-01051-x. View

Light D, Kamm J, Buttery R . Electroantennogram response of alfalfa seed chalcid,Bruchophagus roddi (Hymenoptera: Eurytomidae) to host- and nonhost-plant volatiles. J Chem Ecol. 2013; 18(3):333-52. DOI: 10.1007/BF00994235. View

Dotterl S, David A, Boland W, Silberbauer-Gottsberger I, Gottsberger G . Evidence for behavioral attractiveness of methoxylated aromatics in a dynastid scarab beetle-pollinated araceae. J Chem Ecol. 2012; 38(12):1539-43. DOI: 10.1007/s10886-012-0210-y. View

Tang R, Zhang F, Kone N, Chen J, Zhu F, Han R . Identification and testing of oviposition attractant chemical compounds for Musca domestica. Sci Rep. 2016; 6:33017. PMC: 5036095. DOI: 10.1038/srep33017. View

Li M, Yang Y, Yao Y, Xiang W, Han J, Wang Y . Isolation and identification of attractants from the pupae of three lepidopteran species for the parasitoid Chouioia cunea Yang. Pest Manag Sci. 2019; 76(5):1920-1928. DOI: 10.1002/ps.5724. View

Wang B, Dong W, Li H, DOnofrio C, Bai P, Chen R . Molecular basis of (E)-β-farnesene-mediated aphid location in the predator Eupeodes corollae. Curr Biol. 2022; 32(5):951-962.e7. DOI: 10.1016/j.cub.2021.12.054. View

Li R, Shan S, Song X, Khashaveh A, Wang S, Yin Z . Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator. Int J Biol Macromol. 2022; 223(Pt A):1521-1529. DOI: 10.1016/j.ijbiomac.2022.11.149. View

10.

Bruce T, Pickett J . Perception of plant volatile blends by herbivorous insects--finding the right mix. Phytochemistry. 2011; 72(13):1605-11. DOI: 10.1016/j.phytochem.2011.04.011. View

11.

ONeill B, Zangerl A, DeLucia E, Berenbaum M . Olfactory preferences of Popillia japonica, Vanessa cardui, and Aphis glycines for Glycine max grown under elevated CO2. Environ Entomol. 2011; 39(4):1291-301. DOI: 10.1603/EN09036. View

12.

Osei-Owusu J, Vuts J, Caulfield J, Woodcock C, Withall D, Hooper A . Identification of Semiochemicals from Cowpea, Vigna unguiculata, for Low-input Management of the Legume Pod Borer, Maruca vitrata. J Chem Ecol. 2020; 46(3):288-298. PMC: 7142049. DOI: 10.1007/s10886-020-01149-7. View

13.

Clavijo McCormick A, Unsicker S, Gershenzon J . The specificity of herbivore-induced plant volatiles in attracting herbivore enemies. Trends Plant Sci. 2012; 17(5):303-10. DOI: 10.1016/j.tplants.2012.03.012. View

14.

Zhu J, Park K, Baker T . Identification of odors from overripe mango that attract vinegar flies, Drosophila melanogaster. J Chem Ecol. 2003; 29(4):899-909. DOI: 10.1023/a:1022931816351. View

15.

Zhang Y, Fu X, Wang F, Yang Z . Spatial differences in (Z)-3-hexen-1-ol production preferentially reduces Spodoptera litura larva attack on the young leaves of Nicotiana benthamiana. Plant Sci. 2016; 252:367-373. DOI: 10.1016/j.plantsci.2016.08.016. View

16.

Xu H, Desurmont G, Degen T, Zhou G, Laplanche D, Henryk L . Combined use of herbivore-induced plant volatiles and sex pheromones for mate location in braconid parasitoids. Plant Cell Environ. 2016; 40(3):330-339. DOI: 10.1111/pce.12818. View

17.

Turlings T, Erb M . Tritrophic Interactions Mediated by Herbivore-Induced Plant Volatiles: Mechanisms, Ecological Relevance, and Application Potential. Annu Rev Entomol. 2018; 63:433-452. DOI: 10.1146/annurev-ento-020117-043507. View

18.

Schiestl F . The evolution of floral scent and insect chemical communication. Ecol Lett. 2010; 13(5):643-56. DOI: 10.1111/j.1461-0248.2010.01451.x. View

19.

Xu H, Turlings T . Plant Volatiles as Mate-Finding Cues for Insects. Trends Plant Sci. 2017; 23(2):100-111. DOI: 10.1016/j.tplants.2017.11.004. View

20.

Xiu C, Pan H, Liu B, Luo Z, Williams 3rd L, Yang Y . Perception of and Behavioral Responses to Host Plant Volatiles for Three Adelphocoris Species. J Chem Ecol. 2019; 45(9):779-788. DOI: 10.1007/s10886-019-01102-3. View