HPV16E1 Downregulation Altered the Cell Characteristics Involved in Cervical Cancer Development

Overview

Journal Sci Rep

Specialty Science

Date 2023 Oct 25

PMID 37880374

Authors

Thanayod Sasivimolrattana

Arkom Chaiwongkot

Parvapan Bhattarakosol

Affiliations

Soon will be listed here.

Abstract

The primary causes of cervical cancer are human papillomavirus type 16 (HPV16) and/or other high-risk (Hr -) HPV infections. Hr-HPVE5, E6, and E7 have been identified as oncoproteins that play roles in the development of cancer. However, other HPV proteins, especially E1, may also be involved in cancer development. In this study, the role of HPV16E1 in cervical carcinogenesis was examined by siRNA knockdown experiments using SiHa cells as a model. The results showed that HPV16E1 regulated P-FOXO3a and HPV16E7 expression. Various cell functions associated with the hallmarks of cancer, including cell viability, colony formation, invasion, and anchorage-independent cell growth, were altered when HPV16E1 was downregulated. However, no effect on cell migration and apoptosis properties was found. Moreover, HPV16E1 downregulation resulted in an increase in cisplatin susceptibility. In conclusion, this is the first demonstration that HPV16E1 might be regarded as a possible novel oncoprotein involved in several processes related to oncogenesis.

Citing Articles

Upregulation of HPV16E1 and E7 expression and FOXO3a mRNA downregulation in high-grade cervical neoplasia.

Sasivimolrattana T, Gunawan A, Wattanathavorn W, Pholpong C, Chaiwongkot A, Bhattarakosol P PeerJ. 2024; 12:e18601.

PMID: 39655333 PMC: 11627083. DOI: 10.7717/peerj.18601.

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