Sialic Acid O-acetylation Patterns and Glycosidic Linkage Type Determination by Ion Mobility-mass Spectrometry

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Oct 25

PMID 37880209

Authors

Gajol M Vos

Kevin C Hooijschuur

Zeshi Li

John Fjeldsted

Christian Klein

Robert P de Vries

Javier Sastre Torano

Geert-Jan Boons

Affiliations

Soon will be listed here.

Abstract

O-acetylation is a common modification of sialic acids that has been implicated in a multitude of biological and disease processes. A lack of analytical methods that can determine exact structures of sialic acid variants is a hurdle to determine roles of distinct O-acetylated sialosides. Here, we describe a drift tube ion mobility-mass spectrometry approach that can elucidate exact O-acetylation patterns as well as glycosidic linkage types of sialosides isolated from complex biological samples. It is based on the use of a library of synthetic O-acetylated sialosides to establish intrinsic collision cross section (CCS) values of diagnostic fragment ions. The CCS values were used to characterize O-acetylated sialosides from mucins and N-linked glycans from biologicals as well as equine tracheal and nasal tissues. It uncovered contrasting sialic acid linkage types of acetylated and non-acetylated sialic acids and provided a rationale for sialic acid binding preferences of equine H7 influenza A viruses.

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