Cleave and Rescue Gamete Killers Create Conditions for Gene Drive in Plants

Overview

Journal bioRxiv

Date 2023 Oct 24

PMID 37873352

Authors

Georg Oberhofer

Michelle L Johnson

Tobin Ivy

Igor Antoshechkin

Bruce A Hay

Affiliations

Soon will be listed here.

Abstract

Gene drive elements promote the spread of linked traits, even when their presence confers a fitness cost to carriers, and can be used to change the composition or fate of wild populations. Cleave and Rescue () drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs (the Cleaver/Toxin) that disrupts endogenous versions of an essential gene, and a recoded version of the essential gene resistant to cleavage (the Rescue/Antidote). spreads by creating conditions in which those lacking die because they lack functional versions of the essential gene. We demonstrate the essential features of gene drive in the plant through killing of gametes that fail to inherit a that targets the essential gene YKT61, whose expression is required in male and female gametes for their survival. Resistant (uncleavable but functional) alleles, which can slow or prevent drive, were not observed. Modeling shows plant s are likely to be robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications in plant breeding, weed control, and conservation are discussed.

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