Stem Cell-specific Ecdysone Signaling Regulates the Development and Function of a Sleep Homeostat

Overview

Journal bioRxiv

Date 2023 Oct 24

PMID 37873323

Authors

Adil R Wani

Budhaditya Chowdhury

Jenny Luong

Gonzalo Morales Chaya

Krishna Patel

Jesse Isaacman-Beck

Orie Shafer

Matthew S Kayser

Mubarak Hussain Syed

Affiliations

Soon will be listed here.

Abstract

Complex behaviors arise from neural circuits that are assembled from diverse cell types. Sleep is a conserved and essential behavior, yet little is known regarding how the nervous system generates neuron types of the sleep-wake circuit. Here, we focus on the specification of sleep-promoting neurons-long-field tangential input neurons that project to the dorsal layers of the fan-shaped body neuropil in the central complex (CX). We use lineage analysis and genetic birth dating to identify two bilateral Type II neural stem cells that generate these dorsal fan-shaped body (dFB) neurons. We show that adult dFB neurons express Ecdysone-induced protein E93, and loss of Ecdysone signaling or E93 in Type II NSCs results in the misspecification of the adult dFB neurons. Finally, we show that E93 knockdown in Type II NSCs affects adult sleep behavior. Our results provide insight into how extrinsic hormonal signaling acts on NSCs to generate neuronal diversity required for adult sleep behavior. These findings suggest that some adult sleep disorders might derive from defects in stem cell-specific temporal neurodevelopmental programs.

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