Genome-wide Epigenetic Dynamics During Postnatal Skeletal Muscle Growth in Hu Sheep

Overview

Journal Commun Biol

Specialty Biology

Date 2023 Oct 23

PMID 37872364

Authors

Yutao Cao

Yue Ai

Xiaosheng Zhang

Jinlong Zhang

Xianlei Long

Yaning Zhu

Linli Wang

Qingyi Gu

Hongbing Han

Affiliations

Soon will be listed here.

Abstract

Hypertrophy and fiber transformation are two prominent features of postnatal skeletal muscle development. However, the role of epigenetic modifications is less understood. ATAC-seq, whole genome bisulfite sequencing, and RNA-seq were applied to investigate the epigenetic dynamics of muscle in Hu sheep at 3 days, 3 months, 6 months, and 12 months after birth. All 6865 differentially expressed genes were assigned into three distinct tendencies, highlighting the balanced protein synthesis, accumulated immune activities, and restrained cell division in postnatal development. We identified 3742 differentially accessible regions and 11799 differentially methylated regions that were associated with muscle-development-related pathways in certain stages, like D3-M6. Transcription factor network analysis, based on genomic loci with high chromatin accessibility and low methylation, showed that ARID5B, MYOG, and ENO1 were associated with muscle hypertrophy, while NR1D1, FADS1, ZFP36L2, and SLC25A1 were associated with muscle fiber transformation. Taken together, these results suggest that DNA methylation and chromatin accessibility contributed toward regulating the growth and fiber transformation of postnatal skeletal muscle in Hu sheep.

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