Mechanism of Histone H2B Monoubiquitination by Bre1

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2023 Oct 23

PMID 37872231

Authors

Fan Zhao

Chad W Hicks

Cynthia Wolberger

Affiliations

Soon will be listed here.

Abstract

Monoubiquitination of histone H2B-K120/123 plays several roles in regulating transcription, DNA replication and the DNA damage response. The structure of a nucleosome in complex with the dimeric RING E3 ligase Bre1 reveals that one RING domain binds to the nucleosome acidic patch, where it can position the E2 ubiquitin conjugating enzyme Rad6, while the other RING domain contacts the DNA. Comparisons with H2A-specific E3 ligases suggest a general mechanism of tuning histone specificity via the non-E2-binding RING domain.

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