LncRNA RP11-58O9.2 Predicts Poor Prognosis and Promotes Progression of Non-small Cell Lung Cancer

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2023 Oct 23

PMID 37871619

Authors

Xuan Miao

Wen Xi

Yongxia Bao

Affiliations

Soon will be listed here.

Abstract

Objective: Long non-coding RNAs (lncRNAs) play a crucial role in non-small cell lung cancer (NSCLC). This study aimed to investigate the novel biomarker, lncRNA RP11-58O9.2, in patients with NSCLC.

Methods: RP11-58O9.2 expression in NSCLC cells and tissues was detected by reverse transcription-quantitative polymerase chain reaction. Patient survival was analyzed in relation to RP11-58O9.2 expression levels. RP11-58O9.2 expression was knocked down and endogenous expression was verified in two NSCLC cell lines. Cell proliferation was then assessed by Cell Counting Kit-8 and colony-formation assays, and cell invasion and migration were assessed by Transwell and wound-healing assays, respectively. experiments were performed in mice, and the combination of RP11-58O9.2 and miR-6749-3p was predicted by miRanda.

Results: RP11-58O9.2 was highly expressed in NSCLC cell lines and tissues, and was associated with advanced stage, lymphatic metastasis, and differentiation group. High RP11-58O9.2 levels were also associated with shorter survival. RP11-58O9.2 knockdown inhibited the proliferation, invasion, and migration of lung cancer cells, and tumor growth in mouse xenografts . RP11-58O9.2 may target and regulate miR-6749-3p.

Conclusions: LncRNA RP11-58O9.2 is associated with NSCLC prognosis and promotes lung cancer progression. Further studies are needed to investigate the mechanisms and the regulatory association between RP11-58O9.2 and miR-6749-3p.

Citing Articles

Identifying crucial lncRNAs and mRNAs in hypoxia-induced A549 lung cancer cells and investigating their underlying mechanisms via high-throughput sequencing.

Lin L, Deng L, Bao Y PLoS One. 2024; 19(9):e0307954.

PMID: 39236027 PMC: 11376552. DOI: 10.1371/journal.pone.0307954.

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